The chromosome of Campylobacter jejuni is circular and approximately 1700 kb in circumference. The size of the genome was determined by field inversion gel electrophoresis of restriction endonuclease fragments using lambda DNA concatamers and yeast chromosomes to calibrate the size of the fragments. In view of the low (32-35%) G + C content of the campylobacter genome, enzymes that recognizes GC-rich sequences were used. Of the enzymes tested BssHII (G/C(G)CGC), NciI (CC/CGCG) and SalI (G/TCGAC) appeared to be usable. Hybridization of labeled fragments with two or more fragments from digests with a different restriction enzyme gave the information to order the fragments on the C jejuni chromosome. The localization on the genome of the flagellin and ribosomal gene clusters was determined.
The incubation of trans-3-hexadecenoic acid with cells of Chlorella vulgaris, and with lettuce leaf slices, resulted in reduction of the substrate to palmitic acid.Before this reduction was complete, the trans-acid entered all the acyl lipid classes present in the cell.Consequently, the specific location of trans-3-hexadecenoic in the phosphatidyl glycerol fraction of photosynthetic tissues cannot be explained on the basis that lyso-phosphatidyl glycerol is the only lipid capable of 'accepting' this acid. It is more likely that phosphatidyl glycerol or lyso-phosphatidyl glycerol is a required substrate or cofactor for the synthesis of trans-3-hexadecenoic acid from palmitic acid. . The trans-3-hexadecenoic acid is located at the 2-position of phosphatidyl glycerol [8,10] and the major molecular species of this lipid in spinach has been shown to be l-linolenoyl-2(trans-3-hexadecenoyl)-glycerol-3-phosphoryl-l'-glycerol (nomenclature according to Hirschman [ll]). trans-3-Hexadecenoic acid also occurs in some seed oils [12] but in such cases is presumably associated with the triglyceride fraction.
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