The UDP-2,3-diacylglucosamine pyrophosphate hydrolase LpxH is an essential lipid A biosynthetic enzyme that is conserved in the majority of gram-negative bacteria. It has emerged as an attractive novel antibiotic target due to the recent discovery of an LpxH-targeting sulfonyl piperazine compound (referred to as AZ1) by AstraZeneca. However, the molecular details of AZ1 inhibition have remained unresolved, stymieing further development of this class of antibiotics. Here we report the crystal structure of Klebsiella pneumoniae LpxH in complex with AZ1. We show that AZ1 fits snugly into the L-shaped acyl chain-binding chamber of LpxH with its indoline ring situating adjacent to the active site, its sulfonyl group adopting a sharp kink, and its N-CF3–phenyl substituted piperazine group reaching out to the far side of the LpxH acyl chain-binding chamber. Intriguingly, despite the observation of a single AZ1 conformation in the crystal structure, our solution NMR investigation has revealed the presence of a second ligand conformation invisible in the crystalline state. Together, these distinct ligand conformations delineate a cryptic inhibitor envelope that expands the observed footprint of AZ1 in the LpxH-bound crystal structure and enables the design of AZ1 analogs with enhanced potency in enzymatic assays. These designed compounds display striking improvement in antibiotic activity over AZ1 against wild-type K. pneumoniae, and coadministration with outer membrane permeability enhancers profoundly sensitizes Escherichia coli to designed LpxH inhibitors. Remarkably, none of the sulfonyl piperazine compounds occupies the active site of LpxH, foretelling a straightforward path for rapid optimization of this class of antibiotics.
Dioxygenase
enzymes are essential protein catalysts for the breakdown
of catecholic rings, structural components of plant woody tissue.
This powerful chemistry is used in nature to make antibiotics and
other bioactive materials or degrade plant material, but we have a
limited understanding of the breadth and depth of substrate space
for these potent catalysts. Here we report steady-state and pre-steady-state
kinetic analysis of dopamine derivatives substituted at the 6-position
as substrates of L-DOPA dioxygenase, and an analysis of that activity
as a function of the electron-withdrawing nature of the substituent.
Steady-state and pre-steady-state kinetic data demonstrate the dopamines
are impaired in binding and catalysis with respect to the cosubstrate
molecular oxygen, which likely afforded spectroscopic observation
of an early reaction intermediate, the semiquinone of dopamine. The
reaction pathway of dopamine in the pre-steady state is consistent
with a nonproductive mode of binding of oxygen at the active site.
Despite these limitations, L-DOPA dioxygenase is capable of binding
all of the dopamine derivatives and catalyzing multiple turnovers
of ring cleavage for dopamine, 6-bromodopamine, 6-carboxydopamine,
and 6-cyanodopamine. 6-Nitrodopamine was a single-turnover substrate.
The variety of substrates accepted by the enzyme is consistent with
an interplay of factors, including the capacity of the active site
to bind large, negatively charged groups at the 6-position and the
overall oxidizability of each catecholamine, and is indicative of
the utility of extradiol cleavage in semisynthetic and bioremediation
applications.
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