The RNA methyltransferase NSUN2 has been involved in the cell proliferation and senescence, and is upregulated in various types of cancers. However, the role and potential mechanism of NSUN2 in gastric cancer remains to be determined. Our study showed that NSUN2 was significantly upregulated in gastric cancers, compared to adjacent normal gastric tissues. Moreover, NSUN2 could promote gastric cancer cell proliferation both in vitro and in vivo. Further study demonstrated that CDKN1C (p57 Kip2) was the potential downstream gene of regulated by NSUN2 in gastric cancer. NSUN2 could promote gastric cancer cell proliferation through repressing p57 Kip2 in an m 5 C-dependent manner. Our findings suggested that NSUN2 acted as an oncogene through promoting gastric cancer development by repressing p57 Kip2 in an m 5 C-dependent manner, which may provide a novel therapeutic target against gastric cancer.

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Interactions between smectite and polyelectrolyte play an essential role in exclusive adsorption, flocculation, and thixotropic properties of aqueous smectite/polyelectrolyte system.The materials are widely used in many industries such as drilling fluids, pharmaceuticals, paints and dyes, ceramics, papermaking and water treatment. This review focuses on the interaction between different types of polyelectrolytes and smectite, and their applications in flocculation and industrial wastewaters. The effect of several parameters related to polyelectrolyte such as charge density, charge type and molecular weight, as well as to smectite such as type, particle size, composition, pH and concentration in the aqueous system, were examined. The interactive forces between smectite and polyelectrolyte including hydrophobic interaction, ion binding, hydrogen bonding, van der Waals forces and electrostatic were thoroughly discussed. The interactive forces between smectite and cationic polyelectrolyte, anionic polyelectrolyte and amphoteric polyelectrolyte influence the flocculation of colloidal smectite-polyelectrolyte dispersions such as settling and floc dispersion. However, the interaction between smectites and polyelectrolytes and their subsequent behaviors in adsorption, flocculation, and thixotropy along with inherent mechanisms have not yet fully understood. Future in-depth work on interactions between smectites and polyelectrolytes has great implications for developing functional materials and expanding applications of other polyelectrolyte/ smectite mineral system.

Fabrication of nanochannels is drawing considerable interest due to its broad applications in nanobiotechnology (e.g. biomolecular sensing and single DNA manipulation). Nanochannels offer distinct advantages in allowing a slower translocation and multiple sensing spots along the channel, both of which improve the read-out resolution. However, implementing electrodes inside the nanochannel has rarely been demonstrated to our knowledge. The device described in this work is a Si-Glass anodically bonded Lab-on-a-Chip (LOC) device of a few millimetres in size capable of performing DNA manipulation. The LOC device structure is based on two mainstream microchannels interconnected by nanochannels. DNA, once trapped within the nanochannel, has been tracked throughout the length of the channel and the data have been recorded and analysed.