An improved method for determining the thiobarbituric acid-reactive substances (TBARS) in fish tissue has been described. The biological sample was digested in acidic media and then separated by using specific reflux distillation. The compounds in distillate were estimated by spectrophotometric measurement at 538 nm after the reaction with 2-thiobarbutanic acid. The operational errors, interferences and the recovery of malonaldehyde standard and TBARS from tissue sample using the described procedure have been studied. The recommended technique is simple and reproducible with a detection limit of 0.2 nmol TBARS per 10 g of tissue sample and an overall deviation of less than 7%. The recommended method has been applied satisfactorily for evaluation of rancidity studies in various fish samples.
An investigation for the antioxidative effectiveness of a non-absorbable, polymeric antioxidant, Anoxomer, with the PUFA mackerel skin-model lipids has been evaluated by accelerated oxidation at 60°C. The oxidation rate of the tested samples was mainly followed by measuring the weight gain and changes of TBA value and FFA content. Effectiveness of antioxidation for Anoxomer in the mackerel skin lipids was also compared with other synthetic compounds, BHA, BHT and TBHQ. Overall, the order of effectiveness for inhibiting the oxidation was found to be TBHQ > Anoxomer > BHA > BHT at the concentration of 0.02 % in the tested model lipids. In addition, Anoxomer was also shown to slightly reduce the formation of FFA and carbonyls. The effectiveness of Anoxomer at the level of 0.05-0.10% is recommended for the highly unsaturated lipids and related food products.
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