Summary A total of 57 Indian Sahiwal (Bos indicus) cattle and 53 Murrah and 19 Nili‐Ravi (Bubalus bubalis) buffalo were screened using PCR/RFLP to detect the following alleles at growth hormone (GH) and prolactin (PRL) loci: leucine/valine (L/V) for GH/AluI (amino acid 127), (+/+) for GH/MspI (intron III), and A/B for PRL/RsaI (amino acid 103). Among Sahiwal cattle, gene frequencies were 0.96/0.04 (GH/AluI), 0.86/0.14 (GH/MspI), and 0.49/0.51 (PRL/RsaI). In buffalo, animals of both breeds were homozygous for GH loci (LL, ++). In Egyptian buffalo, 11 samples were also monomorphic, indicating that monomorphism at these two loci may be a species characteristic of buffalo. PRL/RsaI frequencies were 0.93/0.07 and 0.84/0.16 in Murrah and Nili‐Ravi buffalo, respectively. Zusammenfassung Bei insgesamt 57 indischen Sahiwal (Bos indicus) und 53 Murrah und Nili‐Ravi Büffeln (Bubalus bubalis) wurde mit PCR/RFLP auf die folgenden Polymorphismen des Wachstumshormon‐ (GH) und Prolaktingens (PRL) untersucht: L/V für GH/AluI (Austausch Leucin/Valin der Aminosäure 127), (‐/+) für GH/MspI (Intron III) und A/B für PRL/RsaI (Aminosäure 103). Bei Sahiwal waren die Genfrequenzen 0,96/0,04 (GH/AluI), 0,86/0,14 (GH/MspI) und 0,49/0,51 (PRL/RsaI). Bei den Büffeln waren die Tiere beider Rassen — wie auch 11 ägyptische Büffel — homozygot für die GH Loci (LL, ++), was darauf hinweist, daß Büffel monomorph an beiden Schnittstellen sind. Die Frequenzen für PRL/RsaI betrugen 0,93/0,07 und 0,84/0,16 bei den Murrah and Nili‐Ravi Büffeln.

We screened 57 Sahiwal cattle (Bos indicus) and 53 Murrah, 19 Nili-Ravi and 11 Egyptian buffaloes (Bubalus bubalis) to detect the polymorphisms at kappa-casein (CSN3) gene using the polymerase chain reaction (PCR). CSN3 A and B alleles were identified by PCR-RFLP using the restriction enzymes that detect the underlying nucleotide changes at codon 136 (Taql) and at codon 148 (HindIII or HinfI) in cattle. The frequency of CSN3 B allele in the Sahiwal cattle was estimated as 0.16 with no homozygous BB animal. Using the same set of primers as used in the Sahiwal cattle, a part of exon IV of buffalo CSN3 gene was amplified, but restriction enzyme analysis using HindIII/HinfI and TaqI did not reveal any polymorphism. However, DNA sequencing of the amplified fragment (GenBank Acc. No. U96662) revealed one polymorphism at codon 135 (ThrACC -->I1eATC) in buffalo; the frequencies of 135 Thr/Ile alleles were estimated as 0.88 and 0.12, respectively.

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