The objective of this work was to evaluate the efficiency of entomopathogenic nematodes in the control of cassava root mealybug Dysmicoccus sp. under laboratory and greenhouse conditions. Cochineals were reared in “Cabotiá” pumpkin in climatic chambers at 27 ± 1 °C, with relative humidity (RH) of 70 ± 10%, and without photoperiod. The selection test was carried out with 15 isolates, and the ones which caused the greater percentage of insect mortality were used in concentration tests (0, 5, 10, 20, 50 Infective Juveniles/cm²), in sand column displacement, in the in vivo production of Galleria mellonella larvae, and in pathogenicity tests in greenhouse. The isolates NEPET11 (Heterorhabditis sp.) and RSC05 (H. amazonensis) showed the greatest virulence to cochineals in the selection trial, with mortality percentages of 93% and 90%, respectively, and did not differ between each other. In the concentration test, the isolate NEPET11 showed the greatest insect mortality in lower concentrations. With regard to the displacement test, both isolates showed 100% insect mortality, with no significant difference. In the G. mellonella larval production trial of NEPET11 and RSC05 isolates, approximately 7.0 x 104 and 7.2 x 104 infective juveniles/g larvae were produced, respectively, with no significant difference between treatments. Tests in greenhouse pots did not produced significant results.