A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous determination Meropenemand Vaborbactaminpharmaceutical dosage form. The column used was KromosilC18(150mm x 4.6 mm, 5m)in isocratic mode, with mobile phase containing phosphatebufferandacetonitrile (45:55v/v). The buffer is prepared by adding accurately weighed 1.36gm of PotassiumdihyrogenOrtho phosphate in a 1000ml of Volumetric flask add about 900ml of milli-Q water added and degasto sonicate and finally make up the volume with water then pH adjusted to 5.0with dil. Orthophosphoric acid solution. The flow rate was 1.0ml/ min and effluents were monitored at 260nm. The retention times of Meropenemand Vaborbactamwere found to be 2.299 min and 3.102 min, respectively. The linearity for Meropenemand Vaborbactamwere in the range of 25-150μg/mland 25-150 μg/ml respectively. Regression equation of Meropenem is y = 4826.x + 2593, and y = 4887.x + 6194 of Vaborbactamrespectively. The proposed method was validated and successfully applied to the estimation of Meropenemand Vaborbactamin combined tablet dosage forms.
A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous determination Sitagliptinand Etruglifloxinin pharmaceutical dosage form. The column used was Discovery C18(250mm x 4.6 mm, 5m)in isocratic mode, with mobile phase containing phosphatebufferandacetonitrile (45:55v/v). The buffer is prepared by adding accurately weighed 1.36gm of PotassiumdihyrogenOrtho phosphate in a 1000ml of Volumetric flask add about 900ml of milli-Q water added and degas to sonicate and finally make up the volume with water then pH adjusted to 5.4with dil. Orthophosphoric acid solution.The flow rate was 1.0ml/ min and effluents were monitored at 260nm. The retention times of Sitagliptinand Etruglifloxinwere found to be 2.381min and 3.429min, respectively. The linearity for Sitagliptinand Etruglifloxinwere in the range of 25-150μg/mland 3.75-22.5μg/ml respectively. The recoveries of Sitagliptinand Etruglifloxinwere found to be 99.46to 101.19% and 99.36to 100.99%, respectively. The proposed method was validated and successfully applied to the estimation of Sitagliptinand Etruglifloxinin combined tablet dosage forms.
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