The solution of the generalized two‐stream approximation for radiative transfer in homogeneous multiple scattering atmospheres is extended to vertically inhomogeneous atmospheres in a manner which is numerically stable and computationally efficient. It is shown that solar energy deposition rates, photolysis rates, and infrared cooling rates all may be calculated with simple modifications of a single algorithm. The accuracy of the algorithm is generally better than 10% so that other uncertainties, such as in absorption coefficients, may often dominate the error in calculation of the quantities of interest to atmospheric studies.
Metabolic activity was measured in the laboratory at temperatures between 5 and ؊20°C on the basis of incorporation of 14 C-labeled acetate into lipids by samples of a natural population of bacteria from Siberian permafrost (permanently frozen soil). Incorporation followed a sigmoidal pattern similar to growth curves. At all temperatures, the log phase was followed, within 200 to 350 days, by a stationary phase, which was monitored until the 550th day of activity. The minimum doubling times ranged from 1 day (5°C) to 20 days (؊10°C) to ca. 160 days (؊20°C). The curves reached the stationary phase at different levels, depending on the incubation temperature. We suggest that the stationary phase, which is generally considered to be reached when the availability of nutrients becomes limiting, was brought on under our conditions by the formation of diffusion barriers in the thin layers of unfrozen water known to be present in permafrost soils, the thickness of which depends on temperature.In numerous previously published articles the authors described microbial metabolic activity at subzero temperatures. In more recent reviews (6,9,14,22), the authors agree that earlier reports of microbial activity (mostly bacterial activity) at temperatures below Ϫ12°C were unsubstantiated. Microbial growth or metabolic activity has been reported in permafrost bacteria at Ϫ10°C (11) and in the antarctic cryptoendolithic microbial community at temperatures between Ϫ5 and Ϫ10°C (7, 28), and the temperature limit of bacterial growth in frozen food is generally considered to be Ϫ8°C (9). In arctic and antarctic lichens, photosynthetic activity has been observed in a similar temperature range (12) and, more recently, at Ϫ17°C (23). However, no quantitative measurements of the dynamics of metabolic activity or of growth have been described. We attempted to quantify metabolic activity at subzero temperatures in the native bacterial population of Siberian permafrost by measuring the incorporation of sodium acetate into lipids over a 550-day period.Significant numbers of viable bacteria (10 2 to 10 8 cells g Ϫ1 ) are known to be present in permafrost that is 1 to 3 million years old in the arctic (10,21,24,29) 1996, abstr. 60, 1996); all of the bacteria that have been characterized so far have been psychrotrophs (psychrotolerant mesophiles). The ratio of aerobic bacteria to anaerobic bacteria seems to vary according to the geological history. Comparative quantitative studies have not been performed. Permafrost sediments of alluvial, lake, and marine origin that formed under anoxic conditions contain high numbers of anaerobes (compared to total cell counts), like the samples studied by Rivkina et al. (21), whereas other samples, like the samples described by Shi et al. (24) or the sample used in the present study, seem to be dominated by aerobes. Although the exact number of anaerobes in our sample is not known, the anaerobes that were present, which were unable to metabolize under the conditions used in the experiment, obviously did n...
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A combination of culture-dependent and culture-independent methodologies (Bacteria and Archaea 16S rRNA gene clone library analyses) was used to determine the microbial diversity present within a geographically distinct high Arctic permafrost sample. Culturable Bacteria isolates, identified by 16S rRNA gene sequencing, belonged to the phyla Firmicutes, Actinobacteria and Proteobacteria with spore-forming Firmicutes being the most abundant; the majority of the isolates (19/23) were psychrotolerant, some (11/23) were halotolerant, and three isolates grew at -5 degrees C. A Bacteria 16S rRNA gene library containing 101 clones was composed of 42 phylotypes related to diverse phylogenetic groups including the Actinobacteria, Proteobacteria, Firmicutes, Cytophaga - Flavobacteria - Bacteroides, Planctomyces and Gemmatimonadetes; the bacterial 16S rRNA gene phylotypes were dominated by Actinobacteria- and Proteobacteria-related sequences. An Archaea 16S rRNA gene clone library containing 56 clones was made up of 11 phylotypes and contained sequences related to both of the major Archaea domains (Euryarchaeota and Crenarchaeota); the majority of sequences in the Archaea library were related to halophilic Archaea. Characterization of the microbial diversity existing within permafrost environments is important as it will lead to a better understanding of how microorganisms function and survive in such extreme cryoenvironments.
Spectra from Cassini's Visual and Infrared Mapping Spectrometer reveal the presence of a vast tropospheric cloud on Titan at latitudes 51 degrees to 68 degrees north and all longitudes observed (10 degrees to 190 degrees west). The derived characteristics indicate that this cloud is composed of ethane and forms as a result of stratospheric subsidence and the particularly cool conditions near the moon's north pole. Preferential condensation of ethane, perhaps as ice, at Titan's poles during the winters may partially explain the lack of liquid ethane oceans on Titan's surface at middle and lower latitudes.
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