Detection and quantitation of bacillaemia in 50 untreated cases of leprosy were evaluated by the buffy coat method, the haemolysis method and the present Petroff's method. Bacillaemia was detected in 29 (58%) out of 50 cases and in 32 LL-BL cases it was detected in 28 patients with a success rate of 87·5%. Both the haemolysis method and Petroff's method were found useful in estimating the bacillary load per millilitre of blood. Importantly, the smears of concentrated deposit obtained by Petroff's method revealed only AFB free from any artefacts and also yielded high bacterial counts. In conclusion, Petroff's method of concentration was fo und superior over other methods for detection and quantitation of bacillaemia in the lepromatous spectrum (LL-BL) of the disease.Lowe1 appears to be the first worker to observe bacillaemia in leprosy. Subsequently many workers2-6 have demonstrated bacillaemia in various types ofleprosy, especially in the lepromatous spectrum of the disease. Fite7 has fo und leprosy lesions of blood vessels and suggested a continuous shedding of bacilli into circulation. The bacilli have been found in the endothelial cells of blood vessels in the skin and subcutaneous veins of leprosy patients.8-JO Bacillaemia has been detected by various concentration methods. [2][3][4] In the present communication, we have used Petroff's method of concentration for detection as quantitation of bacillaemia in different clinical types of leprosy and the results are compared with those obtained by other concentration methods to evaluate the usefulness of our technique in determining the bacillary load in the blood of a leprosy patient. Material and methodsFifty untreated cases of leprosy consisting of 22 LL, 10 BL, 10 BT and 8 TT patients were investigated. Skin smears from 5 different sites were taken for BI and skin tests using DNeB and 0305-7518/88/059337 +04 SO 1.00
Mal de Meleda is a rare autosomal recessive palmoplantar keratoderma characterized by transgradient keratoderma with associated scleroatrophy, knee changes and onychogryphosis. This case of a 20 year old girl born of second degree consanguineous marriage is reported for its uniqueness in conformity with criteria enunciated by Stulli associated with hyperkeratotic warty papules clinically fitting into Darier's disease with lip involvement. Another interesting feature being black pigmentation of fingers and nails which was due to cashew nut shell paste application mistaken for dry gangrene.
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