HeLa cell nuclei, the reference source for the isolation of factors involved in reactions such as initiation of transcription, splicing, polyadenylation, are obtained following Dounce homogenization of hypotonically fragilized cellsCl• 2, 3). Replacing the homogenization step by nebulization, using the purpose-built BioNeb™, makes it possible to process much larger number of cells than the 2 E + 10 which constitute the maximum daily production. Based on polyacrylamide gel electrophoresis of snRNPs and the evaluation of the splicing activity of nuclear extracts, the quality of nuclei prepared with this instrument compares favourably with the results obtained by douncing.
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