The objective of this study was to investigate the spatiotemporal expression levels and protein localization of extracellular regulated MAP kinase (ERK), HRas proto-oncogene, GTPase (HRAS), and mitogen-activated protein kinase kinase 1 (MEK1)
genes in ovarian prehierarchical follicles of geese. The prehierarchical follicles from healthy laying geese (n=6) at the age of 35 to 37 weeks were harvested. The relative expression levels of ERK, HRAS, and MEK1 in various sized prehierarchical
follicles were detected by real-time quantitative polymerase chain reaction (RT-qPCR), western blotting, and follicular wall localization was investigated by using immunohistochemistry. The results revealed that the candidate genes were expressed
differently at mRNA and protein levels at five stages of prehierarchical follicle development. These results suggest that ERK, HRAS, and MEK1 might be associated to the key biological mechanisms regulating Zi geese folliculogenesis.
The aims of the current study were to investigate the morphological changes in the granulosa cell and theca cell layers and to determine the spatio-temporal expression levels of polyamine-related genes at varying sizes in diameter of the prehierarchical follicles during ovary development. In this current study,H&E staining was used to observe the thickness of the granulosa cells layer and theca cells layer of the prehierarchical follicles, both in which middle white follicles (MWF) were predominantly high. The quantitative mRNA expression of Ornithine decarboxylase (ODC), Ornithine decarboxylase antizyme2 (OAZ2), Spermidine synthase (SRM), Spermine synthase (SMS) and Spermine oxidase (SMOX) was revealed by Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) with different expression levels at each stage of the prehierachical follicular development.The results indicated that polyamine metabolism-related genes were implicated in cell proliferation, differentiation and growth of Zi geese prehierarchical follicles.
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