An experiment of central composite design was performed to determine simultaneously the most suitable concentration of each of three nutrients essential for proliferation of the nematode-trapping fungus,
Arthrobotrys conoides
, and to detect any significant interaction that occurred when they were supplied at different levels. Regression analyses of the results obtained revealed that for maximal growth of
A. conoides
in a glucose-inorganic salts medium, biotin, thiamine, and zinc were required at concentrations of 5, 100, and 400 μg/liter, respectively. No interaction of significance was detected. The analytical procedures are presented in detail, and the benefits that accrue from a statistical approach are discussed.
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