AimsGranulomatous mastitis due to Corynebacterium kroppenstedtii is an increasingly recognised cause of an indolent and distressing mastitis in non-lactating females. This slow-growing lipophilic organism is not reliably isolated using routine culture methods. A novel selective culture medium (CKSM) is designed to optimise the isolation of this organism from clinical specimens.MethodsCKSM contains 10% galactose and Tween 80 (10%) to enhance the growth of C. kroppenstedtii, fosfomycin (100 µg/mL) to suppress the other bacteria, and differentiate C. kroppenstedtii from non-kroppenstedtii lipophilic corynebacteria by esculin hydrolysis. The medium was evaluated for its ability to support the growth of C. kroppenstedtii, selection and differentiation of C. kroppenstedtii from other bacteria in non-sterile clinical specimens.ResultsC. kroppenstedtii grew as 1–2 mm colonies with black halo on CKSM within 72 hours of incubation, compared with barely visible pinpoint colonies on routine blood agars. During the four-month period of evaluation with 8896 respiratory specimens, 103 breast specimens, 1903 female genital tract specimens, 617 newborn surface swabs and 10 011 miscellaneous specimens, 186 C. kroppenstedtii were isolated, including 127 (1.4%) respiratory and 59 (0.5%) miscellaneous specimens, 184 of them were found only on CKSM. Besides the three (2.9%) positive breast specimens, 27 (1.4%) high vaginal and endocervical swabs, and 11 (1.8%) surface swabs of newborns were positive for C. kroppenstedtii.ConclusionsCKSM is a useful addition to routine agar media for the isolation of C. kroppenstedtii, and will be helpful for studying the epidemiology and transmission of this unusual Corynebacterium causing granulomatous mastitis.
The most common pathogens associated with peritonitis in patients on continuous ambulatory peritoneal dialysis (CAPD) are gram-positive bacteria, which constitute 60 - 80% of all isolates. With the advancement of molecular technologies for bacterial identification, cases of CAPD-related peritonitis caused by bacteria previously not known to be associated with this clinical condition have been reported. Here we report the first case of CAPD-related peritonitis due to Laribacter hongkongensis.
globulin were not reported in the paper of Kristiansson et al thyroxine binding globulin deficiency was a constant feature in our patients who presented with a closely related condition.3 The only difference was the clinical course of the condition, which was fatal in both patients (at 7 and 23 months) of Harding et al whereas the patients of Kristiansson et al and those reported by us were all alive (age 3 to 21 years). I therefore recommend a search for defects in glycoprotein metabolism (particularly in the processing and synthesis of the carbohydrate moiety) in patients with olivopontocerebellar atrophy. A valuable screening method for this purpose is the carbohydrate deficient transferrin test devised by Stibler et al.4
We reported the discovery of a novel
Streptococcus
species, propose to be named
Streptococcus oriscaviae
, from the pus collected from a guinea pig bite wound in a healthy young patient. The bacterium was initially misidentified as
S. suis
/
S. parasuis
by biochemical tests, mass spectrometry. and housekeeping genes sequencing.
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