AND C. H. WANG. Dissimilation of glucose and gluconic acid by Pseudomonas natriegens. J. Bacteriol. 83:879-886. 1962-When glucose dissimilation of a marine pseudomonad, Pseudomonas natriegems, was studied, enzymes of both the glycolytic pathway and of the hexose monophosphate pathway were detected in extracts of glucose-grown cells. Enzymes of the Entner-Doudoroff pathway and phosphoketolase were not detected. Data from radiorespirometric experiments indicated that approximately 92 and 8% of glucose actually catabolized were routed via the glycolytic and the hexose monophosphate pathways, respectively. When P. natriegens was induced to utilize gluconate, it was demonstrated that gluconokinase and enzymes of the Entner-Doudoroff pathway were induced. Radiorespirometric experiments with cells under growing conditions revealed that gluconate was dissimilated predominantly (80%) via the Entner-Doudoroff pathway. This observation was in contrast to the
Glucose catabolism in eight Xanthomonas species has been comparatively examined by means of the radiorespirometric method. The basic mechanisms for the respective xanthomonads closely resembled each other. The Entner-Doudoroff pathway, in conjunction with the tricarboxylic acid cycle pathway, was the predominant mechanism for glucose catabolism. A small portion (8 to 16%) of substrate glucose was routed into the pentose phosphate pathway. The hexose cycle pathway did not appear to play any significant role in glucose catabolism of these xanthomonads. The results are also consistent with the well-recognized close phylogenic relationship between xanthomonads and pseudomonads.
Carbohydrate metabolism of the Pseudomonadaceae is known to involve unique catabolic pathways. Lockwood et al. (1941) reported the possible use of Pseudomonas and Phytomonas species for commercial production of gluconate and 2-ketogluconate. Since these compounds are not involved in glycolysis it was apparent that a pathway for glucose degradation other than glycolysis is present in the pseudomonads. Investigations of glucose catabolism by Campbell et al. (1954) and Koepsell (1950) revealed that gluconate, 2-ketogluconate, aketoglutarate, and pyruvate are important intermediates of glucose breakdown in Pseudomonas aeruginosa and Pseudomonas fluorescens. Further insight into the metabolism of this family was gained when Entner and Doudoroff (1952) identified the pathway of glucose catabolism in Pseudomonas saccharophila by means of inhibition and radiotracer studies. Wood (1955) described a similar pathway, which functions concurrently with the pentose phosphate pathway in P. fluorescens. Participation of Entner-Doudoroff and pentose phosphate pathways in pseudomonads were also evaluated by Lewis et al. (1955) and Gibbs and DeMIoss (1954). With the establishment of the pathways of glucose catabolism in pseudomonads, it is therefore of interest to compare the relative participation of individual pathways in the lAided by a grant from the
PLANT PHYSIOLOGY when r = 1.5 cm and k = 0.0025 cm2/sec. To use this system the apparatus was modified to record either v1 (as usual) or the difference, v1 -v2. As v1 is recorded the output is occasionally switched to (vl -v2), thus checking that the ratio v1/v2 is constant with time. The value of k is found using the previous formulae, and used in equation 16 to obtain V cos 6.
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