Summary-A microparticle-enhanced nephelometric immunoassay of bovine immunoglobulins G is reported. lt is based on the nephelometric quantification of the competitive immunoagglutination of covalently coated microparticles. This new immunoassay is easy to perform (1-step, no sample pretreatment, no washing or phase separation), fast (1 h at most), sensitive (8 Ilgl1 of immunoglobulins G significantly detected in the reaction mixture), accu rate (linear recovery of immunoglobulins G in overloaded milks) and reproducible (CVs from 2.3-10% in within-and between-run precision studies). The potential of this microparticle-based immunoassay ls discussed with regard to the other quantitative immunoassays (radial immunodiffusion and convention al immunonephelometry in parneular) used in the determination of milk immunoglobulins G.
We have studied the pharmacokinetics of tiopronin and its principal metabolite, 2-mercaptopropionic acid (2-MPA) in healthy volunteers after the oral administration of 500 mg (2 Acadione tablets), followed by simultaneous assay of the two compounds in plasma over a period of 48 h using a new method (emission of fluorescence after HPLC and post-column derivatization by pyrene-maleimide). The absorption of tiopronin was slow (tmax between 4 and 6 h) and the plasma concentrations subsequently fell biexponentially. The principal metabolite 2-MPA appeared later in the plasma (tmax between 10 and 12 h after a lag-time of 3 h) then disappeared monoexponentially. About 15% of the tiopronin was metabolized to 2-MPA.
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