The protein constituents of serum can range from grams to picograms per liter, making it technically difficult to achieve in-depth proteomic analysis. Removal of highly abundant proteins, such as albumin, coupled to powerful protein separation methods is required for increased sample load, thus facilitating detection and identification of low-abundant proteins. We report here a chemical-based extraction method for the effective and specific removal of albumin from serum.
We have developed a new detector for Escherichia coli and coliform bacteria that can be used on site with automated signal analysis. Fluorogenic "targeted substrates" and a fibre-optic detector monitor glucuronidase and galactosidase enzymes as indicators for E. coli and Total Coliform, respectively. The test has been certified by AOAC and approved for use in laboratories by the Ontario Ministry of the Environment. The test has been demonstrated on samples from drinking water to sewage, with no change in the analysis method and no need for dilutions. Single organisms were detected in about 13 h, with highly contaminated samples detected earlier. The bacteria were quantified using the continuous signal and a model for growth kinetics. Sample-to-sample repeatability was similar to plate-counting methods. The calibration function for a new site or type of bacteria can be adjusted using a small number of samples.The bacteria tests for Escherichia coli (EC) and total coliforms (TC) are arguably the most important tests for indicating the safety of drinking water. E. coli (EC) is also the main parameter used to indicate the microbiological quality of source water and water at various points in the treatment process. Conventional E. coli tests are mainly done in laboratories, either because of regulatory requirements or WEFTEC 2010
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