We therefore believe that FLIM has a potential future clinical role in imaging BCCs for rapid and noninvasive tumour delineation and as an aid to determine adequate excision margins with best preservation of normal tissue.

No abstract

The combination of MPT and FLIM may be useful for the in vitro study of cell modifications induced by injurious or protective agents and drugs.

A fluorescence lifetime imaging (FLIM) system is described that utilizes a new compact and low-cost ultrafast laser source based on a gain-switched laser diode-seeded all-solid-state Cr:LiSAF regenerative amplifier that has been designed for this application. The pulse parameters of this source (0.5 μJ, 827 nm, 100 ps, 5 kHz) are shown to be appropriate to time-domain FLIM using a gated optical intensifier and the application to functional imaging of biological tissue is demonstrated, as well as the first evaluation of organic light emitting diodes using FLIM.

The sections in this article are Introduction Autofluorescence of Biological Tissue Fluorescence Contrast Spectroscopic Techniques for Fluorescence Imaging and Metrology Spectral Techniques Fluorescence Lifetime Techniques Single‐Point Spectroscopic Techniques Fluorescence Microscopy Laser Scanning Confocal/Multiphoton Microscopy Wide‐Field Fluorescence Imaging Multidimensional Fluorescence Microscopy Ex Vivo Tissue Spectroscopy and Imaging: Application to Cancer Oral Cavity Gastrointestinal Tract Bladder Breast Brain Skin Conclusion Acknowledgments

No abstract