This brief review is focused on different methodologies available for detection of anti-dsDNA antibodies with respect to the best adequacy between biological results of laboratory and clinical significance. A large array of assays has been developed for the measurement of anti-dsDNA. New assays continually introduced have reflected not only technical innovations to avoid difficulties of some assays, but even more with hope to correlate better with systemic lupus erythematosus (SLE), particularly with its clinical course and exacerbations. Finally, with the development of micro arrays technology new insights into the pathophysiology of autoimmune disease processes should be revealed.
Our study was done to evaluate the FIDIS Connective kit (Biomedical Diagnostics, Marne la Vallée, France) for simultaneous quantitative determination in the same sample of 9 antinuclear antibody specificities directed against double-stranded DNA, SSA, SSB, Sm, Sm/RNP, Scl-70, Jo-1, ribosome, and centromere B and to compare it with standardized commercial methods, enzyme immunoassay and immunofluorescence. FIDIS technology constitutes a new multiplexed method using the Luminex 100 system (Luminex, Austin, TX) based on the use of distinct color-coded particles and flow cytometric detection. Serum samples from people with diagnosed rheumatic diseases with well-identified markers of autoimmunity were tested by a retrospective study. Specificity was assessed by testing blood donors and potential biologic interfering samples. This first evaluation demonstrated the analytic performance of FIDIS technology. Concordances with routine methods were between 99.1% and 100.0% on 222 samples. FIDIS was reliable (coefficients of variation < 10%) and accurate (correlation coefficients with enzyme-linked immunosorbent assay between 0.90 and 0.97) in a large measure range.
Our study was done to evaluate the FIDIS Connective kit (Biomedical Diagnostics, Marne la Vallée, France) for simultaneous quantitative determination in the same sample of 9 antinuclear antibody specificities directed against double-stranded DNA, SSA, SSB, Sm, Sm/RNP, Scl-70, Jo-1, ribosome, and centromere B and to compare it with standardized commercial methods, enzyme immunoassay and immunofluorescence. FIDIS technology constitutes a new multiplexed method using the Luminex 100 system (Luminex, Austin, TX) based on the use of distinct color-coded particles and flow cytometric detection. Serum samples from people with diagnosed rheumatic diseases with well-identified markers of autoimmunity were tested by a retrospective study. Specificity was assessed by testing blood donors and potential biologic interfering samples. This first evaluation demonstrated the analytic performance of FIDIS technology. Concordances with routine methods were between 99.1% and 100.0% on 222 samples. FIDIS was reliable (coefficients of variation < 10%) and accurate (correlation coefficients with enzyme-linked immunosorbent assay between 0.90 and 0.97) in a large measure range.
Two brothers with infantile myofibromatosis are reported. Both had cutaneous and skeletal myofibromas with spontaneous and complete healing of their cutaneous lesions. These cases suggest autosomal recessive inheritance of this rare disorder.
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