We present a method to determine where the nanoparticles nucleate and grow during pulsed laser deposition in an ambient gas. Briefly, nanocrystalline Si films are systemically deposited on the substrates located at a distance from the plasma and placed in horizontal direction; meanwhile an external electric field is introduced perpendicularly to the plume. Based on the transportation dynamics of Si nanoparticles corresponding to different electric fields, the lateral nucleation range of 0.1 to 33.8 mm is determined for Si nanoparticles deposited in 10 Pa Ar gas at a laser fluence of 4 J/cm 2 . Further simulation of the mass and area density of Si nanoparticles demonstrates that both nucleation and growth probabilities in nucleation region are approximately Gauss-dependent of the lateral distance.
MP [4-(3′,3′-dimethylallyloxy)-5-methyl-6-methoxyphthalide] was obtained from liquid
culture of Pestalotiopsis photiniae isolated from the Chinese
Podocarpaceae plant Podocarpus macrophyllus. MP significantly
inhibited the proliferation of HeLa tumor cell lines. After treatment with MP,
characteristic apoptotic features such as DNA fragmentation and chromatin
condensation were observed in DAPI-stained HeLa cells. Flow cytometry showed that MP
induced G1 cell cycle arrest and apoptosis in a dose-dependent manner. Western
blotting and real-time reverse transcription-polymerase chain reaction were used to
investigate protein and mRNA expression. MP caused significant cell cycle arrest by
upregulating the cyclin-dependent kinase inhibitor p27KIP1 protein and
p21CIP1 mRNA levels in HeLa cells. The expression of p73 protein was
increased after treatment with various MP concentrations. mRNA expression of the cell
cycle-related genes, p21CIP1, p16INK4a and Gadd45α, was significantly upregulated and mRNA levels
demonstrated significantly increased translation of p73,
JunB, FKHR, and Bim. The
results indicate that MP may be a potential treatment for cervical cancer.
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