Studies on seventy-five sera containing antibodies of the H‘0’I-B complex together with a survey of studies by other workers show that many of the antibodies formerly called anti-‘0’ and some of those called anti-H are in fact reacting with a joint product of the H and I genes. The serological specificities of these antibodies were shown by agglutination tests with cells of known H and I or B and I status, together with the use of neutralisation and absorption techniques. These tests revealed that many of the sera contain mixtures of antibody specificities. The occurrence of anti-H-HI antibodies is probably associated with pregnancy. An H notation based on that proposed by Wiener et al. is recommended for use in the classification of these antibodies.
An evaluation is given of haemolysin, neutralisation, thermostability, saline titration, anti-A® antibody absorption and 2-mercapto-ethanol studies on the sera from 178 clinically affected ABO HD cases and 130 control cases. The antiglobulin titre on the 2-mercapto-ethanol treated sera which determines the IgG titre was the best procedure used for the demonstration of significant immune anti-A/B antibodies in the maternal sera. The screen series on 5,704 sera from group 0 mothers for the detection of immune anti-A/B and the attempted prediction of ABO HD revealed that ABO HD occurs with a frequency of about 0.8%. Exchange transfusion was needed in six cases i.e. about 0.1% of all the infants of group 0 mothers screened. The minimum criteria for the diagnosis of ABO HD are the serological demonstration of incompatible anti-A/B antibodies in an eluate prepared from the baby’s red cells, accompanied by the clinical observation of jaundice, or more rarely pallor due to anaemia, in the infant during the first few days following birth. In the absence of elution or serum studies on a blood sample from the baby, the presence of an incompatible high titre (>256) IgG antibody in the maternal serum provides good presumptive evidence that the baby may be suffering from ABO HD.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.