Purpose
To examine a relationship between serum transforming growth factor beta-1 (TGF-β1) values and radiation induced fibrosis (RIF).
Methods
We conducted a prospective analysis of development of RIF in 38 women with AJCC Stage 0-I breast cancer treated with lumpectomy and accelerated partial breast irradiation via interstitial brachytherapy (IBAPBI). An ELISA (Quantikine ®, R&D, MN) immunoassay was used to measure serum TGF-β1 pre-surgery, pre- IBAPBI, and during IBAPBI. Blood samples for TGF-β1 were also collected from 15 healthy, non-treated women (controls). The previously validated tissue compliance meter (TCM) was used to objectively assess radiation-induced fibrosis (RIF).
Results
Median time to follow-up for 38 patients is 44 months (range, 5 – 59 months). RIF is graded by the TCM scale as 0, 1, 2 and 3 in 5/20 (25%), 6/20 (30%), 5/20 (25%) and 4/20 (20%) patients, respectively. ΔTCM ≥6mm (moderate-to-severe RIF) is statistically different from ΔTCM ≤3mm (none-to-mild RIF) (p<0.05). Mean serum TGF-β1 values are significantly higher in patients pre-surgery than disease-free controls in: a) all cancer patients (30,201 ± 5,889 pg/ml, p=0.02), b) patients with any type of RIF (32,273 ± 5,016 pg/ml, p<0.0001) and c) women with moderate-to-severe RIF (34,462 ± 4,713 pg/ml, p<0.0001). The post-IBAPBI mean serum TGF-β1 is 21,915pg/ml in patients with ΔTCM ≥6mm (moderate-severe RIF). This is significantly higher than the mean serum TGF-β1, 14,940pg/ml, in patients with ΔTCM ≤3mm (p = 0.036). In patients who develop moderate-to-severe RIF, pre-IAPBI mean TGF-β1 values are also predictive of this sequela (17,885 ± 3,952 pg/ml, p=0.007).
Conclusions
TGF-β1 levels correlate with development of moderate-to-severe RIF. The pre-IBAPBI mean TGF-β1 levels can serve as an early biomarker for development of moderate-to-severe RIF after IBAPBI