C o n t r i b u t i o n s to the C y t o l o g y of I n d i a n S c o r p i o n sThe . This is due to hypocoiling of the chromosomes. While t h e possibility of colchicine inducing constrictions u n r e l a t e d to kinetochore exists, the revealing of unseen constrictions is of interest. The c h r o m o s o m e b e h a v i o u r in Bulhus tamulus of S o u t h I n d i a is unique because, in the testicular cells of t h e s a m e individual, the following t h r e e t y p e s of orientat i o n a t m e t a p h a s e I and a n a p h a s e I are e n c o u n t e r e d : (1) t h e axes of c h r o m o s o m e s are at r i g h t angles to t h e equatorial plate, suggesting m o n o c e n t r y ( Figure d) ; (2) the axes of c h r o m o s o m e s are parallel to t h e e q u a t o r i a l axis, suggesting diffused or holokinetic n a t u r e ( Figure h); a n d (3) t h e crescentic a p p e a r a n c e of chromosomes, suggesting b i c e n t r y (Figure i). The crescentic n a t u r e of c h r o m o s o m e s of B. tamulus of Mysore S t a t e are a m e n a b l e for interpret a t i o n as inonocentric, bicentric, a n d hotokinetic. This o b s e r v a t i o n w a r r a n t s a r e a s s e s s m e n t of our opinion n o t only of kinetochore b u t also the axis relationships as criteria for designation of c h r o m o s o m e types. This is especially so in the light of t h e o b s e r v a t i o n s of RHOADES and VELKOMERSON 16 on p l a n t chromosomes, in w h i c h t h e y h a v e s h o w n 'neocentric' a c t i v i t y of n o n -c e n t r o m e r i e regions of the c h r o m o s o m e . F u r t h e r , c h r o m o s o m e s of B. tamulus d e s i g n a t e d as m o n o c e n t r i c c a n n o t be reconciled w i t h t h e fact t h a t t h e r e is a higher degree of v a r i a b i l i t y i n t e r p r e t a b l e only b y breakage and r e t e n t i o n of chromos o m e bits in t h e k a r y o t y p e . This o b s e r v a t i o n can be explained only on the basis of t h e holokinetic n a t u r e of t h e c h r o m o s o m e s . Zusammen[assung.Die C h r o m o s o m e n z a h l von Heterometrus scaber ist 96. Wird Palamnaeus swammerdami m i t Colchicin (0,2%) b e h a n d e l t , so ziehen sich die Chromos o m e n in der Pr~imetaphase I z u s a m m e n . Buthus lamulus (aus Mysore) zeigt w e i t g e h e n d variable C h r o m o s o m e n z a h l (20 28). Die L a g e der C h r o m o s o m e n in der M e t a p h a s e I und A n a p h a s e i weist auf Monozentrie, 13izentrie u n d Holokinetie.
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