Aim: This study was performed to isolate and characterize novel antifungal lipopeptide from Bacillus cereus. Methods and Results: Elucidation of its chemical structure was carried out by electrospray ionization mass spectra (ESI-MS) and Fourier transform infrared spectroscopy (FT-IR). The compound is a cyclic heptapeptide and composed of amino acids, Leu-Asp-Val-Leu-Leu-Leu-Leu. The in vitro activity of Kannurin against various pathogenic yeasts was assessed by CLSI M27-A and moulds by M38-A. It demonstrated broad-spectrum, fungicidal activity against clinically relevant yeasts and moulds. Kannurin exhibited low haemolytic activity and remained active over a wide pH and temperature range. In addition, Kannurin did not bind with melanin particles and was as active in inhibiting biofilms. Conclusions: An antifungal surfactin-like lipopeptide produced by Bacillus cereus strain AK1 was purified and chemically characterized. We propose to name this lipopeptide compound 'Kannurin'. To our knowledge, this is the first report of Bacillus cereus producing surfactin-like lipopeptide antibiotic with stronger antifungal activity. Significance and Impact of the Study: Our results provide a valuable contribution towards a better understanding of the lipopeptide of Bacillus cereus. Moreover, it raises the possibility of using as an alternative antibiotic in clinical medicine.
A copolymer of 1,6-hexanediol diacrylate (HDODA) and styrene was prepared by a suspension polymerization method. The resin was characterized by infrared and carbon-13 cross-polarization magic-angle spin ( 13 C CP-MAS) spectroscopy. The topology of the resin was examined by scanning electron microscopy (SEM). The polymer swells extensively in common solvents used for peptide synthesis. The resin exhibited chemical stability even in neat trifluoroacetic acid. The applicability of the new resin was demonstrated by synthesis of Val-Ala-Val-Ala-Ala-Gly, Gln-ValGly-Gln-Val-Glu-Leu-Gly, and Val-Gln-Ala-Ala-Ile-AspTyr-Ile-Asn-Gly. Comparative synthetic studies showed that the new resin is superior to divinylbenzene (DVB)-based resin in the case of the synthesis of hydrophobic peptide sequences.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.