Polyploidy is a biotechnology that in the last decade had provided with an improvement method for mollusks in aquaculture. Triploids have three sets of chromosomes rather than the two carried by diploids. The third set has an effect on chromosomes synapsis, producing sterility during reproduction. That sterility is the most accepted cause for the better growth seen in triploids. Artificial induction to triploidy has some problems, which can be solved by the existence of tetraploids, which could be mated to diploids to produce biological triploids. Control strategies based on prevention of disease and methods that slow the spread of such diseases. Therefore, proper management of diseases affecting the tomato crop, knowledge and understanding of the diagnosis and its infectious cycle is vital and to establish effective control measures.However, the literature is limited when it comes to auscultating information related with the poliploidía in mollusks, it´s origin and the multiple biotechnologies that develop contemporary. This study was conducted to assess and meet the trend of existing research on polyploidy in commercially important shellfissh.
Triploid Crassostrea gigas were cultured during 13 months in Nestier-type oyster trays. The impact of environmental parameters on the physiological and immunological parameters was evaluated. Temperature, salinity and seston were recorded monthly. Seventeen oysters were sampled monthly for immunological and condition index (CI) analyses. Samples were obtained as a haemolymph lysate supernatant (HLS). Protein content was determined using the Bradford method. The activity of hydrolytic enzymes was determined using the API ZYM kit and the lysoplate assay. Seston showed di¡erent patterns throughout the cycle. Condition index showed a positive correlation with the protein content of HLS. Protein showed a negative correlation with temperature. Eleven hydrolytic enzymes were detected in samples and higher enzymatic activity corresponded to leucine arylamidase and esterase. Leucine arylamidase and lysozyme activity showed a positive correlation with temperature. Oyster mortality was 28% in our modules and 70% in the oyster farm. Oysters showed low values of CI and haemolymph protein content in summer^autumn when mortalities were observed in the culture system. This ¢nding suggests that these stressed oysters may have insu⁄cient energy to invest in their immune system. It appears that oyster mortality in the culture system resulted from a combination of animal overcrowding, high temperature and low salinity.
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