During the three hours required for absorption of most of a U-C-14-glucose load (1 gm./kg.) given orally to an intact unanesthetized dog in the postabsorptive state, about three quarters of the load reaches the peripheral circulation. The absence of randomization of the label, seen when an equal oral load of 6-C-14-glucose is given, indicates that the glucose reaching the peripheral circulation has not been fragmented, then resynthesized into glucose. During the three-hour period, only 0.34 gm. per kilogram of new (unlabeled) glucose is released by liver instead of the 0.63 gm. per kilogram which would have been produced in the absence of oral glucose; the uptake of the glucose of peripheral circulating blood is 1.03 gm. per kilogram instead of 0.63 gm. per kilogram. Endogenous insulin secreted in response to the oral load exerts a somewhat larger effect to decrease hepatic glucose release (relative to the increase in glucose uptake from peripheral blood) than is the case when insulin is given by peripheral vein. DIABETES 17:415-21, July, 1968.The glucose tolerance test, in which a glucose load is administered orally or intravenously, continues to serve as a useful indicator of abnormalities in glucose metabolism. The changes in blood glucose concentration which form the basis for the interpretation of the test are influenced by several factors besides the rate of uptake of the glucose load by the tissues.The "K value" for the intravenous test, which appears to represent the velocity constant of a first order disappearance process in which glucose concentration determines the rate of glucose uptake by the tissues, is most likely a hybrid number. The early fall in blood glucose concentration after intravenous glucose injection is hastened by the spreading of the injected load throughout the glucose space. This is evidenced

Recent studies proposed that [2T]glucose is preferable to [14C]-glucose as a tracer for the measurement of glucose turnover. However, higher values for glucose turnover were obtained using [2T]glucose than with [14C]glucose. The present study explores the merit of another species of tritiated glucose, [3T]glucose. Utilizing isotope-dilution principles, comparison is made of glucose turnover values determined by use of [2T]glucose, [3T]glucose, and [6-14C]glucose. Glucose turnover using [2T]glucose was 1.51 +/- 0.07 times greater than that using [6-14C]glucose, after correction for recycling of 14C. However, glucose turnover values obtained with [3T]glucose were similar to those obtained with [6-14C]glucose. There were no temporal or quantitative differences in appearance of tritium (T) in plasma water after injection of [2T]- and [3T]glucose. A methylprednisolone regimen in the normal dog increased glucose turnover as determined by all three tracers, but the increase observed using [2T]glusoce was significantly greater than that using that two other tracers. Thiement for [6-14C]glucose for measurement of glucose turnover in the dog.

329bile, but the intestines showed no color that would indicate the presence of bile.The results of the experiment are shown in Table I. It is apparent that 0.1 mg of bile salts per gram body weight resulted in the appearance of a much greater degree of hemorrhage in the fasted animals, and that even one-tenth this amount had a definite effect.Summary. 1. Sudden reduction of atmospheric pressure to 70 mm Hg caused pulmonary hemorrhage and death, in mice. 2. Fasting had the effect of reducing the degree of hemorrhage. 3. Bile salts, given tc the fasted animals increased the degree of hemorrhage.Goldmanl observed that trypan blue intravenously injected localized in parts ot the placenta and in certain of the foetal membranes of mice, b,ut not in the embryos. These observations were in general confirmed by Burrows2 and by Everett? Paralleling these reports many studies have indicated that colloidal acid-am dyes in the same class as trypan blue, when injected intravenmsly, localize in traumatized areas, as well as in certain induced or naturally-occurring lec' pions. Among these studies was the dbservation that such dyes localize in neoplasms.Zahl and Waters4 made a histological study of the localization of various dyes in mouse sarcoma 180, extending similar earlier observations by Ludford? Duran-Reynals? Brunschwig, Schmitz and Clarke? In the course of this study it was observed that certain bacterial products would, when injected 1 Goldman, E. E., Beitr. z. Llin.

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