The active multilayer packaging films were formed from low-density polyethylene (LDPE) and polyamide containing a 2% antimicrobial agent in one of the LDPE sides of the film (LDPE/polyamide/LDPE-2% antimicrobial agent). The antimicrobial agents used were potassium sorbate (PS-film), nisin (N-film), or chitosan (CTS-film). The effects of antimicrobial incorporation on water vapor permeability (P), diffusivity (D eff ), and solubility (S o and S H ) of the active and control films (LDPE/ polyamide/LDPE) were investigated. A dynamic vapor sorption analyzer (DVS) was used to estimate the sorption isotherms of the films at 25 °C. Peleg was found to be the best equation to describe sorption behaviors. The addition of PS and nisin into the film matrix resulted in a lower P than that of the control film. The D eff values of the active films were lower than those of control films, except for the CTS-film. The high water-holding capacity of PS and nisin might limit the D eff of the respective films. It was found that Henry's law was applicable to relate P, D eff , and S o and S H values of the multilayer film [correlation coefficient (r) = 0.909-0.971]. The mechanical and thermal properties of the active films were not significantly affected by the incorporation of PS and nisin (p [ 0.05). However, the impact of stress and elongation (transverse direction) on the CTS-film was lower than on other films, which indicated that chitosan improved the mechanical properties of the film.
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