The applicability of using odourless glycosidically bound volatiles (GBVs) as fragrance materials was investigated. After incubating skin microflora with various kinds of GBVs, the amounts of fragrance materials generated and the remaining glycosides were analysed by GC and HPLC. Most GBVs, particularlyˇ-D-glucosides, were metabolized by various skin microflora to release their aglycones as fragrance materials. It was assumed that this was due to differences in enzymatic activity of the microflora. The results also suggest their considerable usefulness as alternative fragrance materials.
A simple turbidimetric method was developed to detect alginate degradation. Bacteria were grown in alginate-containing media, and culture fluids were mixed with an acidic albumin solution. Failure to develop a white turbidity indicated an alginate degrader. The method showed alginate degradation by Vibrio alginolyticus ATCC 17749, in contrast to prior descriptions.
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