17CpG Methylation of polynucleotides is one of the most studied epigenetic mechanisms that enable 18 organisms to change their phenotype without altering the genotype. More recently CpG methylation 19 occurring on small noncoding RNAs, especially of certain transfer RNAs has come into focus. This 20 modification is established by the most conserved member of the DNA methyltransferase family, 21Dnmt2. 22 2 downregulation and slowed down the development in the offspring larvae. Although, we could not 35 observe a significant paternal priming effect independent of treatment, paternal knockdown led to 36 increased mortality after bacterial injection with B. thuringiensis. 37This demonstrates again an increased stress sensitivity caused by a lack of Dnmt2. Furthermore, to the 38 best of our knowledge this is the first instance where this effect was observed in the offspring 39 generation. In conclusion, our results highlight the importance of Dnmt2 and show the need to further 40 investigate this enzyme and its function in tRNA methylation and paternal non-genetic inheritance. 41 42
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