The increase of Zika virus (ZIKV) infections in Brazil in the last two years leaves a prophylactic measures on alert for this new and emerging pathogen. Concerning of our positive experience, we developed a new prototype using Neisseria meningitidis outer membrane vesicles (OMV) on ZIKV cell growth in a fusion of OMV in the envelope of virus particles. The fusion of nanoparticles resulting from outer membrane vesicles of N. meningitidis with infected C6/36 cells line were analyzed by Nano tracking analysis (NTA), zeta potential, differential light scattering (DLS), scan and scanning transmission eletronic microscopy (SEM and STEM) and high resolution mass spectometry (HRMS) for nanostructure characterization. Also, the vaccination effects were viewed by immune response in mice protocols immunization (ELISA and inflammatory chemokines) confirmed by Zika virus soroneutralization test. The results of immunizations in mice showed that antibody production had a titer greater than 1:160 as compared to unvaccinated mice. The immune response of the adjuvant and non-adjuvant formulation activated the cellular immune response TH1 and TH2. In addition, the serum neutralization was able to prevent infection of virus particles in the glial tumor cell model (M059J). This research shows efficient strategies without recombinant technology or DNA vaccines.

Resumo Zika virus (ZIKV) é um membro da família Flaviviridae que causa doença febril aguda e outras complicações em humanos. ZIKV foi isolado no final da década de 40 na África mas continuou desconhecido de grande parte da população até causar uma grande epidemia na Polinésia Francesa em 2013. O primeiro caso autóctone de Zika no Brasil aconteceu no início de 2015 e desde então diversas doenças diferentes foram supostamente associadas à infecção por esse vírus.O diagnóstico laboratorial é feito através da reação em cadeia da polimerase precedida de transcrição reversa em tempo real que identifica a presença do material genético do vírus no soro do paciente.

Ambos os patógenos são de grande importância para a epidemiologia global atualmente, uma vez que as meningites bacterianas são as causadoras de dezenas de milhares de mortes anualmente, sendo uma das bactérias causadoras de meningite a Neisseria meningitidis e a infecção de gestantes pelo Zika Virus (ZIKV) está relacionado com o nascimento de crianças com microcefalia (OMS, 2016), assim como possui relação com a síndrome de Guillain-Barré, bem como a possibilidade de infecção por troca de fluídos, como a amamentação, por exemplo.

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The fifty-two bacteria were isolated from urinary tract infections in University Hospital in Sumaré, São Paulo State, Brazil.  These isolates were analyzed about their antibiotic resistance and their bacterial characteristics. Around percentage from these isolates were identified as belonging to Escherichia coli strains, followed by Klebsiella pneumoniae and Pseudomonas aeruginosa. The multiresistance profile (resistance profile major than four 4 antibiotic classes) were viewed in thirteen (13) strains of Escherichia coli, sixteen (16) strains in Klebsiella pneumoniae (being two (2) carbapenem resistant suggesting an existence of KPC strain). The results had demonstrated an indiscriminate antibiotic use concentrated in the increase of quinolones resistance (principally in ciprofloxacin use), and also the rise of KPC super bacteria.

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Several vaccine prototypes were made using variations in concentration, volume, time and speed of agitation. From these prototypes, some characterization assays were carried out, such as the analysis of particle size and zeta potential, where it was possible to determine the size of the nanoparticles, the polydispersity index, with the most promising samples having a size of 252.1nm ± 71.2 nm. The polydispersity index was 0.572 ±0.02, the standard deviation being a little high due to the presence of free viral particles and some associated vesicles. It is noteworthy that the isolated vesicle has a size of 140 to 160nm and the polydispersity index of 0.513 demonstrates that we do not have interference in the sample of our vesicle (it is known that the size of the viral particle of Zika virus is between 40 and 60nm). Cytotoxicity testing was done to analyze whether they have cytotoxic profiles and also demonstrate that the prototype's neutralization and inactivation method works correctly. The analysis of the protein profile of some of the samples revealed that there are two major Zika virus proteins in the fused proteasome, namely the E protein, which is directly involved in the recognition of host cell receptors, and the C protein, which is related to capsid assembly, in addition to playing an important role in the immune response.