BackgroundAging is associated with complex and constant remodeling of the immune function, resulting in an increasing susceptibility to infection and others diseases. The infections caused by Gram-negative microorganisms, present in nursing homes and hospitals, constitute one of the most common infections in the elderly, and are mainly combated by innate immune cells. Although the functions of innate immunity seem more preserved during aging than of adaptive immune mechanisms, two systems operate in an integrated way in the body, so that injury in one part of the immune system inevitably affects the other as they are part of a defensive network. The aim of this study was to investigate the in vitro production of proinflammatory (TNF-α, IL-6, IL-1β, CXCL-8 and MCP-1) and anti-inflammatory (TGF-β and IL-10) cytokines by monocytes, stimulated or not (basal) with lipopolysaccharide, from healthy young and elderly subjects. By means of PBMCs, we also studied if cytokine profile is altered in these different patient groups, in the presence of lymphocytes, under the same experimental conditions.ResultsThe monocytes from elderly presented higher basal production of TNF-α, MCP-1 and lower of TGF-β than young monocytes. PBMC showed similar cytokines production, irrespective age or stimulation presence. In the presence of lymphocytes, the spontaneous production of IL-10 was higher and of TGF-β was lower than monocytes, regardless of age. After LPS-stimulation, the presence of lymphocytes resulted in increased IL-6, IL-1β, MCP-1 and IL-10 and decreased CXCL-8 and TGF-β in comparison to pure culture of monocytes from young patients. With age, the same differences were observed, except for CXCL-8 and TGF-β which production was the same between monocytes and PBMC stimulated with LPS.ConclusionThese findings reinforce the systemic state of inflamm-aging frequently reported in elderly and considered a factor of susceptibility to numerous diseases. Still, the cytokine production from just monocytes of the elderly showed alterations, while in the lymphocyte presence not, suggesting an immunomodulator role of lymphocytes on monocytes. In addition, the differences between the production patterns by LPS-stimulated PBMC between young and elderly volunteers can be related with an imbalance in response against Gram-negative bacteria in throughout life.
Fonte de financiamento: CAPES ARTIGO ORIGINAL RESUMO | Contexto: A Odontologia do Trabalho é responsável por estudar, interpretar e solucionar os diferentes problemas bucais dos trabalhadores, contribuindo para sua qualidade de vida e maior produtividade. Objetivos: Avaliar as condições bucais de trabalhadores em duas fábricas de baterias e o uso de serviços odontológicos em Bauru, São Paulo. Métodos: A amostra foi constituída por 134 indivíduos que responderam a um questionário sobre o uso de serviços. Os exames foram realizados utilizando-se o Índice CPOD para a cárie dentária, Índice Periodontal Comunitário (IPC) e Perda de Inserção Periodontal (PIP) para a doença periodontal, e o Índice de Desgaste Dentário (IDD) (Kappa>0,94). A análise foi descritiva, por meio de frequências absolutas e relativas. Resultados: Com relação ao uso de serviços, 55,22% relataram consulta odontológica há menos de 1 ano, 76,87% buscaram atendimento particular/convênio e 33,58% como consulta de rotina. Apenas 12,60% dos trabalhadores apresentaram-se livres de cárie, sendo observado CPOD médio de 8,49. Houve presença de cálculo em 56,70%, bolsas rasas em 17,90% e bolsas profundas em 1,50% dos trabalhadores. A perda de inserção periodontal foi observada em 26,52%. Todos os trabalhadores apresentaram desgaste dentário, sendo esse mais prevalente em dentina. Conclusão: Os serviços odontológicos oferecidos pelas empresas pesquisadas propiciaram uma condição bucal satisfatória aos trabalhadores. Palavras-chave | odontologia do trabalho; saúde do trabalhador; serviços de saúde do trabalhador.ABSTRACT | Context:Occupational Dentistry is responsible for studying, interpreting and solving the various oral problems of workers, contributing to their quality of life and greater productivity. Objectives: To evaluate the oral conditions of workers in two battery factories and the use of dental services in Bauru, São Paulo, Brazil. Methods: The sample consisted of 134 individuals who responded to a questionnaire on the use of services. The tests were conducted using the DMFT index for dental caries, Community Periodontal Index (CPI) and Periodontal Attachment Loss (PAL) for periodontal disease, and the Dental Wear Index (DWI) (Kappa>0.94). A descriptive analysis was done by means of absolute and relative frequencies. Results: Regarding the use of services, 55.22% reported dental visits in less than 1 year, 76.87% sought private care, and 33.58% as part ofa routine consultation. Only 12.60% of the workers were free of caries, with mean DMFT of 8.49. There was calculus in 56.70%, shallow pockets in 17.90% and deep pockets in 1.50% of the workers. The periodontal attachment loss was observed in 26.52%. All workers had tooth wear, which was more prevalent in dentin. Conclusion: The dental services offered by the surveyed companies provided a satisfactory oral health to the workers.
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