Stoichiometric homeostasis of heterotrophs is a common, but not always well-examined premise in ecological stoichiometry. We experimentally evaluated the relationship between substrate (plant litter) and consumer (microorganisms) stoichiometry for a tropical terrestrial decomposer system. Variation in microbial C : P and N : P ratios tracked that of the soluble litter fraction, but not that of bulk leaf litter material. Microbial N and P were not isometrically related, suggesting higher rates of P than N sequestration in microbial biomass. Shifts in microbial stoichiometry were related to changes in microbial community structure. Our results indicate that P in dissolved form is a major driver of terrestrial microbial stoichiometry, similar to aquatic environments. The demonstrated relative plasticity in microbial C : P and N : P and the critical role of P have important implications for theoretical modelling and contribute to a process-based understanding of stoichiometric relationships and the flow of elements across trophic levels in decomposer systems.
HPLC analyses revealed that caffeoylquinic acid content was 10-fold lower in adult than in juvenile leaves. The most abundant cinnamate conjugate was 5-CQA, but dicaffeoylquinic acids (particularly in juvenile leaves) and feruloylquinic acids were also present. Using specific reagents, histochemical and microspectrofluorometrical analysis showed that caffeoylquinic acids (mono- and di-esters) were closely associated with chloroplasts in very young leaves. During leaf ageing, they were found to first accumulate intensively in specific chlorenchymatous bundle sheath cells and then in phloem sclerenchyma cells. The association with chloroplasts suggests that caffeoylquinic acids have a protective role against light damage. In older tissues, their presence in the leaf vascular system indicates that they are transported via phloem and confirms their involvement in lignification processes. In accordance with the hypothesis of a complex formation with caffeine, similar tissue distribution was observed for alkaloids and this is further discussed.
1. In the fig (Moraceae) and fig-wasp (Agaonidae) mutualism, scent is believed to be of primary importance in pollinator attraction and maintenance of species specificity. Scent divergence between closely related Ficus species seems sufficient in promoting reproductive isolation through pollinator behaviour, starting the process of speciation. 2. We investigated volatile organic compound (VOC) variation from figs in several Ficus species endemic to Papua New Guinea. Sister species of section Papuacyse and subspecies of Ficus trichocerasa substitute each other along the continuously forested Mt. Wilhelm elevational gradient. We placed these species in a phylogenetic context to draw conclusions of scent divergence between close relatives. In addition, pollinator response to VOCs emitted by figs of different species was tested. 3. Volatile profiles differed significantly between focal species, although with a varying degree of overlap between (sub)species and elevations. Pollinators were generally attracted to VOCs emitted only by their hosts except in one case where pollinating fig wasps were also attracted to the sister species of its host. Wasp morphological traits, however, indicate that it is mechanically impossible for this species to oviposit in figs of this atypical encounter. 4. Synthesis. This study demonstrates that while scent is an effective signal for partner recognition, there are multiple barriers which help maintain prepollination isolation in fig and pollinating fig-wasp interactions. Speciation along this elevational gradient is reinforced by divergence in key reproductive isolation mechanisms on both sides of the mutualism. K E Y W O R D S character divergence, evolutionary ecology, fig pollination, fig-wasp attraction, reproductive isolation, sister species, speciation, volatile organic compounds (VOCs) | 2257 Journal of Ecology SOUTO-VILARÓS eT AL.
Many species show changes in distribution and phenotypic trait variation in response to climatic warming. Evidence of genetically based trait responses to climate change is, however, less common. Here, we detected evolutionary variation in the landscape-scale distribution of a genetically based chemical polymorphism in Mediterranean wild thyme (Thymus vulgaris) in association with modified extreme winter freezing events. By comparing current data on morph distribution with that observed in the early 1970s, we detected a significant increase in the proportion of morphs that are sensitive to winter freezing. This increase in frequency was observed in 17 of the 24 populations in which, since the 1970s, annual extreme winter freezing temperatures have risen above the thresholds that cause mortality of freezing-sensitive morphs. Our results provide an original example of rapid ongoing evolutionary change associated with relaxed selection (less extreme freezing events) on a local landscape scale. In species whose distribution and genetic variability are shaped by strong selection gradients, there may be little time lag associated with their ecological and evolutionary response to longterm environmental change.
Little is known on aphid-induced emissions of volatile organic compounds (VOCs) from trees and particularly on their intraspecific variability in association with resistance traits. We compared VOC emissions from five peach cultivars (Prunus persica (L.) Batsch) and a wild relative (Prunus davidiana (Carrière) Franch) that differ in their level (susceptible/resistant) and type (antixenosis, antibiosis) of resistance to the green peach aphid, Myzus persicae (Sulzer). Additionally, the kinetics of VOC induction in response to aphids was compared with that by mechanical wounding. Qualitative and overall quantitative differences among peach genotypes were found in VOC emissions that were mainly composed of methyl-salicylate, farnesenes, (E)-β-ocimene and (E)-4,8-dimethyl-1,3,7-nonatriene. Irrespective of the type of resistance, all resistant genotypes had increased VOC emissions upon aphid attack, while in susceptible genotypes emissions remained low. Emission increases were highest in the genotypes that express increased aphid resistance during second infestations, which had also the highest proportions of methyl-salicylate in their emissions. VOC induction by aphids proceeded slowly with a delay of several hours. Artificial wounding of leaves did not result in emissions of aphid-induced VOCs but caused an immediate burst of green leaf volatiles and benzaldehyde. We conclude that VOC induction in resistant peach cultivars is part of a general defence syndrome that is being avoided or suppressed by M. persicae in the susceptible genotypes. The induction likely involves an aphid-specific elicitor and (methyl)-salicylate in the subsequent signalling and regulation processes that should include gene activation due to the marked delay in the emission response. The results are compared with those of the literature and discussed in view of their ecological and environmental significance.
Summary 1.Interactions between entomophilous plants and their pollinators are one of the major factors shaping the evolution of floral features. As species are distributed in more or less connected populations, they have evolved in a geographical mosaic of co-evolution were the outcome of the plant-pollinator interaction is likely to vary as a result of local adaptations. 2. Arum italicum and Arum maculatum are two species of Araceae which deceive their fly pollinators by mimicking the odour of their oviposition sites. Whereas A. italicum is known to be pollinated by flies belonging to different families (i.e. opportunist), A. maculatum relies on only two pollinating species of the family Psychodidae throughout its European repartition area (i.e. specialist). 3. The interannual and geographical variations of pollinators and pollinator-attractive odours were described in several populations of the two species over two consecutive years. Furthermore, local adaptation to pollinators was tested by transplanting inflorescence-bearing plants between two different sites and by recording the number and composition of the insect fauna trapped inside the inflorescences during anthesis as a measure of a fitness component. 4. Pollinators and pollinator-attractive odours of the two Arum species varied in time and space, but there was no clear odour structure between populations. When transplanted, inflorescences of both species trapped the same composition and number of insects as native inflorescences at a given site; this indicates that pollinator composition is highly dependent on the local availability of insects. 5. No pattern of local adaptation was found for these two species, but local pollination conditions were shown to strongly affect the degree of geographical variations of these interactions. The lack of a clear odour geographical structure might be due to high gene flow or to similar selective pressures exerted by pollinators, and the high interindividual odour variation may be linked to the deceptive strategy adopted by the two plant species.
In several highly specialized plant-insect interactions, scent-mediated specificity of pollinator attraction is directed by the emission and detection of volatile organic compounds (VOCs). Although some plants engaged in such interactions emit singular compounds, others emit mixtures of VOCs commonly emitted by plants. We investigated the chemical ecological bases of host plant recognition in the nursery pollination mutualism between the dioecious Ficus carica and its specific pollinator Blastophaga psenes. Using Y-tube olfactometer tests, we show that B. psenes females are attracted by VOCs of receptive figs of both sexes and do not exhibit preference for VOCs of either male or female figs. Electrophysiological tests and chemical analysis revealed that of all the VOCs emitted by receptive figs, only five were found to be active on female antennae. Behavioural tests show that, in contrast to VOCs presented alone, only a blend with a particular proportion of four of these VOCs is as attractive as the odour of receptive figs, and that if there is a very small change in this blend proportion, the pollinator is no longer attracted. This study revealed that in highly specialized mutualistic interactions specificity could be mediated by a particular blend of common compounds emitted by plants.
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