Group A rotaviruses (RVAs) are leading causes of viral diarrhea in children and in the young of many animal species, particularly swine. In the current study, porcine RVAs were found in fecal specimens from symptomatic piglets on 4 farms in Brazil during the years of 2012-2013. Using RT-PCR, Sanger nucleotide sequencing, and phylogenetic analyses, the whole genomes of 12 Brazilian porcine RVA strains were analyzed. Specifically, the full-length open reading frame (ORF) sequences were determined for the NSP2-, NSP3-, and VP6-coding genes, and partial ORF sequences were determined for the VP1-, VP2-, VP3-, VP4-, VP7-, NSP1-, NSP4-, and NSP5/6-coding genes. The results indicate that all 12 strains had an overall porcine-RVA-like backbone with most segments being designated as genotype 1, with the exception of the VP6- and NSP1-coding genes, which were genotypes I5 and A8, respectively. These results add to our growing understanding of porcine RVA genetic diversity and will provide a platform for monitoring the role of animals as genetic reservoirs of emerging human RVAs strains.
A identificação de parâmetros/ métricas associados à adaptação ambiental é uma das principais tendências e desafios da pecuária mundial. Desta forma, o objetivo deste trabalho foi avaliar as características de tolerância ao calor de bovinos da raça Nelore e Pantaneiro no ecossistema do Pantanal matogrossense no pico de calor da região. Estes animais foram mantidos por duas horas à sombra (8:00h às 10:00h) e em seguida uma hora ao sol (10:00h às 11:00h). Após a exposição foram analisados parâmetros de frequência cardíaca (FC), frequência respiratória (FR), temperatura retal (TR), temperatura superficial da costela (TSC), temperatura superficial do pescoço (TSP) e temperatura superficial da garupa (TSG). Também foram avaliados a taxa de sudação (TXS), comprimento e densidade de pelos e calculados o índice de tolerância ao calor (ITC). No período estudado, a temperatura ambiental variou de 29,0 a 40,0ºC e a média para umidade relativa foi de 75%. O ambiente teve influência marcante sobre as variáveis fisiológicas, com exceção da FR. A TR e a TXS diferiram significativamente entre os grupos genéticos, sendo da raça Nelore mais elevada em relação à Pantaneira, mas todas dentro dos limites fisiológicos. A análise multivariada mostrou a importância de variáveis como taxa de sudação e comprimento de pelos para adaptação de bovinos na região. Apesar do Nelore vermelho estar separado dos outros dois grupos, foi possível concluir que ambas as raças analisadas apresentaram capacidade de adaptação e tolerância ao calor às condições climáticas do Pantanal e podem ser produzidas de modo sustentável na região.
Rotaviruses are a major cause of diarrhea in humans and animals, including several mammalian and avian species. Using different PCR protocols, we report the occurrence of rotavirus A in 21 (53.84%; 21/39) from 39 fecal pool samples of broilers, layers, and broiler breeders from Brazilian avian farms. We typed the G5, G8, G11, G19, and P[31] genotypes.
Rotaviruses are responsible for the acute diarrhea in various mammalian and avian species. The nonstructural proteins NSP2 and NSP5 are involved in the rotavirus replication and the formation of viroplasm, cytoplasmic inclusion bodies within which new viral particles morphogenesis and viral RNA replication occur. There are few studies on the genetic diversity of those proteins; thus this study aims at characterizing the diversity of rotavirus based on NSP2 and NSP5 genes in rotaviruses circulating in Brazilian pig farms. For this purpose, 63 fecal samples from pig farms located in six different cities in the São Paulo State, Brazil, were screened by nested RT-PCR. Seven strains had the partial nucleotide sequencing for NSP2, whereas in six, the total sequencing for NSP5. All were characterized as genotype H1 and N1. The nucleotide identity of NSP2 genes ranged from 100% to 86.4% and the amino acid identity from 100% to 91.5%. For NSP5, the nucleotide identity was from 100% to 95.1% and the amino acid identity from 100% to 97.4%. It is concluded that the genotypes of the strains circulating in the region of study are in agreement with those reported in the literature for swine and that there is the possibility of interaction between human and animal rotaviruses.
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