Timed artificial insemination (TAI) has boosted the use of conventional artificial insemination (CAI) by employing hormonal protocols to synchronize oestrus and ovulation. This study aimed to evaluate the efficiency of a hormonal protocol for TAI in mares, based on a combination of progesterone releasing intravaginal device (PRID), prostaglandin (PGF2α) and human chorionic gonadotropin (hCG); and compare financial costs between CAI and TAI. Twenty‐one mares were divided into two groups: CAI group (CAIG; n = 6 mares; 17 oestrous cycles) and TAI group (TAIG; n = 15 mares; 15 oestrous cycles). The CAIG was subjected to CAI, involving follicular dynamics and uterine oedema monitoring with ultrasound examinations (US), and administration of hCG (1,600 IU) when the dominant follicle (DF) diameter's ≥35 mm + uterine oedema + cervix opening. The AI was performed with fresh semen (500 × 106 cells), and embryo was recovered on day 8 (D8) after ovulation. In TAI, mares received 1.9 g PRID on D0. On D10, PRID was removed and 6.71 mg dinoprost tromethamine was administered. Ovulation was induced on D14 (1,600 IU of hCG) regardless of the DF diameter's, and AI was performed with fresh semen (500 × 106 cells). On D30 after AI, pregnancy was confirmed by US. The pregnancy rate was 80.0% in TAIG and 82.3% in CAIG (p > .05). The TAI protocol resulted in 65% reduction in professional transport costs, and 40% reduction in material costs. The TAI was as efficient as CAI, provided reduction in costs and handlings, and is recommended in mares.
Tendo em vista que afecções do sistema respiratório são grandes responsáveis por perdas de rendimento atlético de equinos, faz-se necessário o diagnóstico e tratamento precoce da doença para que o animal possa retornar à sua rotina sem perder de forma drástica sua performance. O presente artigo tem como objetivo abordar a técnica de toracoscopia como método diagnóstico e auxiliar no tratamento da pleuropneumonia em equinos, bem como relatar o caso de um cavalo com histórico de febre intermitente não responsiva à tratamentos com antibióticos e antipiréticos, sinais clínicos compatíveis com pleuropneumonia e diagnóstico confirmado pela ultrassonografia torácica. O exame toracoscópico bilateral foi essencial para observação e lavagem da cavidade torácica por meio de um dreno de silicone, e é sabido que a colocação de drenos torácicos com auxílio da toracoscopia resulta em alto índice de recuperação atlética dos animais. Desta forma, a toracoscopia vem como uma grande auxiliadora para a clínica de equinos, pois permite que o médico veterinário, além de ter uma visão mais completa da cavidade torácica e detalhes específicos do caso, também possa usar este método de exame complementar no auxílio ao tratamento pela drenagem da efusão pleural e fixação de dreno, garantindo melhora clínica do animal e rápida recuperação.
The study aimed to compare two techniques for plasma semen removal from 11 stallions using semen centrifugation and semen filtration. Two hundred and thirteen semen samples were frozen and evaluated. The samples were distributed into two groups: centrifuged semen group (GSC) and filtered semen group (GSF). The semen was evaluated fresh and after freezing based on sperm movement (vigor) and sperm motility. After the analysis of the fresh ejaculate, the samples were submitted to centrifugation (600 g/10 minutes) or filtration through synthetic hydrophilic membrane SpermFilter and were frozen. Significant differences were found for the characteristics of motility (P = 0.0002) and vigor (P = 0.0221) between GSC and GSF groups in the pre-freezing time. Sperm motility was observed in 74.6 % (GSC) and 79.4 % (GSF) in pre-freezing time. After freezing phase, sperm motility resulted in 63.4 % (GSC) and 65.8 % (GSF) (P = 0.2303); and vigor showed a reduction in the number of samples classified for score 4, and a predominance of the samples classified in scores 2 and 3 in both groups with no difference between groups. It was concluded that the different methods of seminal plasma removal used (centrifugation or filtration) did not influence sperm quality after freezing; the semen filtration was better in the pre-freezing moment showing the best technique for the use of fresh semen.
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