Abstract. By taking advantage of a lethal phenotype characteristic of Caenorhabditis elegans embryos that fail to move, we have identified 13 genes required for muscle assembly and function and discovered a new lethal class of alleles for three previously known muscle-affecting genes. By staining mutant embryos for myosin and actin we have recognized five distinct classes of genes: mutations in four genes disrupt the assembly of thick and thin filaments into the myofilamerit lattice as well as the polarized location of these components to the sarcolemma. Mutations in another three genes also disrupt thick and thin filament assembly, but allow proper polarization of lattice components based on the myosin heavy chain isoform that we analyzed. Another two classes of genes are defined by mutations with principal effects on thick or thin filament assembly into the lattice, but not both. The final class includes three genes in which mutations cause relatively minor defects in lattice assembly. Failure of certain mutants to stain with antibodies to specific muscle cell antigens suggest that two genes associated with severe disruptions of myofilament lattice assembly may code for components of the basement membrane and the sarcolemma that are concentrated where dense bodies (Z-line analogs) and M-lines attach to the cell membrane. Similar evidence suggests that one of the genes associated with mild effects on lattice assembly may code for tropomyosin. Many of the newly identified genes are likely to play critical roles in muscle development and function.

Our data strengthens the emerging view that ILK functions primarily as an adaptor protein within integrin adhesion complexes and identifies UNC-112 as a new ILK binding partner.

The present study investigated whether the assumptions of an ideal point response process, similar in spirit to Thurstone's work in the context of attitude measurement, can provide viable alternatives to the traditionally used dominance assumptions for personality item calibration and scoring. Item response theory methods were used to compare the fit of 2 ideal point and 2 dominance models with data from the 5th edition of the Sixteen Personality Factor Questionnaire (S. Conn & M. L. Rieke, 1994). The authors' results indicate that ideal point models can provide as good or better fit to personality items than do dominance models because they can fit monotonically increasing item response functions but do not require this property. Several implications of these findings for personality measurement and personnel selection are described.

Mutations in the unc-52 gene of Caenorhabditis elegans affect attachment of the myofilament lattice to the muscle cell membrane. Here, we demonstrate that the unc-52 gene encodes a nematode homolog of perlecan, the mammalian basement membrane heparan sulfate proteoglycan. The longest potential open reading frame of this gene encodes a 2482-amino-acid protein with a signal peptide and four domains. The first domain is unique to the unc-52 polypeptide, whereas the three remaining domains contain sequences found in the LDL receptor (domain II) laminin (domain III) and N-CAM (domain IV). We have identified three alternatively spliced transcripts that encode different carboxy-terminal sequences. The two larger transcripts encode proteins containing all or part of domain IV, whereas the smaller transcript encodes a shortened polypeptide that completely lacks domain IV. We have determined that the disorganized muscle phenotype observed in unc-52(st196) animals is caused by the insertion of a Tcl transposon into domain IV. Two monoclonal antibodies that recognize an extracellular component of all contractile tissues in C. elegans fail to stain embryos homozygous for a lethal unc-52 allele. We have mapped the epitopes recognized by both monoclonal antibodies to a region of domain IV in the unc-52-encoded protein sequence.

This study examined how well current software implementations of four polytomous item response theory models fit several multiple-choice tests. The models were Bock's (1972) nominal model, Samejima's (1979) multiple-choice Model C, Thissen & Steinberg's (1984) multiple-choice model, and Levine's (1993) maximum-likelihood formula scoring model. The parameters of the first three of these models were estimated with Thissen's (1986) MULTILOG computer program; Williams & Levine's (1993) FORSCORE program was used for Levine's model. Tests from the Armed Services Vocational Aptitude Battery, the Scholastic Aptitude Test, and the American College Test Assessment were analyzed. The models were fit in estimation samples of approximately 3,000; cross-validation samples of approximately 3,000 were used to evaluate goodness of fit. Both fit plots and X2 statistics were used to determine the adequacy of fit. Bock's model provided surprisingly good fit; adding parameters to the nominal model did not yield improvements in fit. FORSCORE provided generally good fit for Levine's nonparametric model across all tests. Index terms: Bock's nominal model, FORSCORE, maximum likelihood formula scoring, MULTILOG, polytomous IRT.