Sunimar>'. A mathematical model is presenied which may be applied to describe and analyse daia from microscopic phagocyiosis assays. The meihod has been used to investigate the phagocytosis of opsonized yeast by peripheral blood neutrophils Ireatcd with purified recombinam human granulocyte-macrophage colony-stimulating factor {rH GM-CSF) m vitro. Under limiting conditions of scrum opsonization, rH GM-CSF decreased the proportion of non-phagocyiic cells and increased the mean number of ingested yeast per cell. Slimulation of phagocytosis was dose-dependent and occurred with concentrations of rH GM-CSF in the range 10-320 uniis/ml. The effect was dependent on a heat-labile component in serum and was not attributable to endotoxin contamination of the preparation.

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A computer controlled ventilation system was employed to maintain a constant end-expired CO2 percentage ( F E CO2) during a series of studies on young dogs aimed at measuring brain temperature differences during surface cooling hypothermia. The conclusion is that an F E CO2 of six per cent or more is desirable if brain temperature differences are to be minimized. Additional experiments showed that ethyl alcohol and sodium nitroprusside infusions during cooling did not decrease brain temperature differences.

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