Bacteriophage (phage) are bacterial viruses and are considered to be the most widely distributed and diverse natural biological entities. Soon after their discovery, bacteriophage were found to have antimicrobial properties that were exploited in many early anti-infection trials. However, the subsequent discovery of antibiotics led to a decline in the popularity of bacteriophage in much of the Western world, although work continued in the former Soviet Union and Eastern Europe. As a result of the emergence of antibiotic resistance in a number of bacterial pathogens, focus has been redirected back to bacteriophage and bacteriophage lysins as a means of pathogen control. Although bacteriophage have certain limitations, significant progress has been made toward their applications in food and has resulted in the U.S. Food and Drug Administration approving the use of a bacteriophage-based additive for the control of Listeria monocytogenes contamination. Furthermore, a number of animal studies have revealed the potential of bacteriophage for the control of various foodborne pathogens within the animal gastrointestinal tract and to subsequently decrease the likelihood of foodborne outbreaks. From a biopreservative perspective, phage have a number of key properties, including relative stability during storage, an ability to self-replicate, and a nontoxic nature. The purpose of this review is to highlight the recent developments in the use of phages and their lysins for biocontrol and to address their potential future applications.
This study investigated the effect of bacteriophages (phages) e11/2 and e4/1c against Escherichia coli O157:H7 in an ex vivo rumen model and in cattle in vivo. In the ex vivo rumen model, samples were inoculated with either 10 3 or 10 6 CFU/ml inoculum of E. coli O157:H7 and challenged separately with each bacteriophage. In the presence of phage e11/2, the numbers of E. coli O157:H7 bacteria were significantly (P < 0.05) reduced to below the limit of detection within 1 h. Phage e4/1c significantly (P < 0.05) reduced E. coli O157:H7 numbers within 2 h of incubation, but the number of surviving E. coli O157:H7 bacteria then remained unchanged over a further 22-h incubation period. The ability of a phage cocktail of e11/2 and e4/1c to reduce the fecal shedding of E. coli O157:H7 in experimentally inoculated cattle was then investigated in two cattle trials. Cattle (yearlings, n ؍ 20 for trial one; adult fistulated cattle, n ؍ 2 for trial two) were orally inoculated with 10 10 CFU of E. coli O157:H7. Animals (n ؍ 10 for trial one; n ؍ 1 for trial two) were dosed daily with a bacteriophage cocktail of 10 11 PFU for 3 days postinoculation. E. coli O157:H7 and phage numbers in fecal and/or rumen samples were determined over 7 days postinoculation. E. coli O157:H7 numbers rapidly declined in all animals within 24 to 48 h; however, there was no significant difference (P > 0.05) between the numbers of E. coli O157:H7 bacteria shed by the phage-treated or control animals. Phages were recovered from the rumen but not from the feces of the adult fistulated animal in trial two but were recovered from the feces of the yearling animals in trial one. While the results from the rumen model suggest that phages are effective in the rumen, further research is required to improve the antimicrobial effectiveness of phages for the elimination of E. coli O157:H7 in vivo.
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