The purpose of this systematic review was to investigate previously published literature that examined the impact of acculturative stress on health in Spanish-speaking populations. The database search yielded a final count of 32 eligible articles for inclusion in this review. Age, acculturative stress measure, and study results were analyzed. Overall, 81.2% of studies investigated the impacts of acculturative stress on mental health with the majority concluding that increased acculturative stress had a significant relationship with greater mental health issues, such as depression, anxiety, and psychological stress. Other studies in this review examined impacts on physical health and health behaviors, but yielded inconclusive results indicating that these are areas for future research.