. HABEEB, and DESAIS G. TUCK. Can, J. Chem. 55. 1527Chem. 55. (1977. The X-ray photoelectron spectra (metal and halogen energy levels) have been recorded for binary halides of indium in the +I; +II, and t I11 states, and for anionic halide corilplexes of indium (1) and (111). The results are internally consistent in terms of the relation between metal and ligand levels. but lattice effects prevent any detailed interpretation of indium levels in terms of oxidation state, ligand electronegativity, andjor coordination number.
Lactic acid bacteria (LAB) are capable of synthesising metabolites known as exopolysaccharides (EPS) during fermentation. Traditionally, EPS plays an important role in fermented dairy products through their gelling and thickening properties, but they can also be beneficial to human health. This bioactivity has gained attention in applications for functional foods, which leads them to have prebiotic, immunomodulatory, antioxidant, anti-tumour, cholesterol-lowering and anti-obesity activity. Understanding the parameters and conditions is crucial to optimising the EPS yields from LAB for applications in the food industry. This review provides an overview of the functional food market together with the biosynthesis of EPS. Factors influencing the production of EPS as well as methods for isolation, characterisation and quantification are reviewed. Finally, the health benefits associated with EPS are discussed.
Biological reaction calorimetry, also known as biocalorimetry, has led to extensive applications in monitoring and control of different bioprocesses. A simple real-time estimator for biomass and growth rate was formulated, based on in-line measured metabolic heat flow values. The performance of the estimator was tested in a unique bench-scale calorimeter (BioRC1), improved to a sensitivity range of 8 mW l(-1) in order to facilitate the monitoring of even weakly exothermic biochemical reactions. A proportional-integral feedback control strategy based on these estimators was designed and implemented to control the growth rate of Candida utilis, Kluyveromyces marxianus and Pichia pastoris by regulating an exponential substrate feed. Maintaining a particular specific growth rate throughout a culture is essential for reproducible product quality in industrial bioprocesses and therefore a key sequence for the step from quality by analysis to quality by design. The potential of biocalorimetry as a reliable biomass monitoring tool and as a key part of a robust control strategy for aerobic fed-batch cultures of Crabtree-negative yeast cells in defined growth medium was investigated. Presenting controller errors of less than 4% in the best cases, the approach paves the way for the development of a generally applicable process analytical technology platform for monitoring and control of microbial fed-batch cultures.
With increasing pressure from regulatory authorities on industry to develop processes embracing process analytical technology (PAT) initiatives, there is a growing demand to establish reliable tools and systems capable of meeting this need. With regard to monitoring and control of bioprocesses, this need translates to a search for robust instrumentation capable of monitoring the critical process parameters in real time. The application of such technologies at all stages of the process, from the initial R&D phase to process optimisation and production, enhances process understanding and paves the way for the development of control platforms. An examination of the PAT concept and selected tools (NIR, MIR, Raman, dielectric spectroscopy and calorimetry) are presented here. A description of each tool is given, with particular emphasis on the nature of the signal produced and how these relate to measurements of biomass, metabolites and product. A description of the signal processing that is necessary to gain meaningful results from the different tools is also given, together with online data reconciliation techniques based on mass and energy balances. Many techniques such as those based on vibrational spectroscopy are of particular interest, since they are capable of monitoring several critical process parameters which are typically controlled in a bioprocess. A window of application for each of the techniques, when used in the area of bioprocessing, is suggested based on their uses and inherent limitations.
Every year, the EU emits 13.4 Mt of CO2 solely from plastic production, with 99% of all plastics being produced from fossil fuel sources, while those that are produced from renewable sources use food products as feedstocks. In 2019, 29 Mt of plastic waste was collected in Europe. It is estimated that 32% was recycled, 43% was incinerated and 25% was sent to landfill. It has been estimated that life-sciences (biology, medicine, etc.) alone create plastic waste of approximately 5.5 Mt/yr, the majority being disposed of by incineration. The vast majority of this plastic waste is made from fossil fuel sources, though there is a growing interest in the possible use of bioplastics as a viable alternative for single-use lab consumables, such as petri dishes, pipette tips, etc. However, to-date only limited bioplastic replacement examples exist. In this review, common polymers used for labware are discussed, along with examining the possibility of replacing these materials with bioplastics, specifically polylactic acid (PLA). The material properties of PLA are described, along with possible functional improvements dure to additives. Finally, the standards and benchmarks needed for assessing bioplastics produced for labware components are reviewed.
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