Water is the most important nutrient in animal nutrition; however, water intake is rarely measured. The objective of this study was to determine whether previously published water intake (WI) equations for beef cattle would accurately predict WI from four experiments conducted under tropical conditions. The experiments were conducted from 2013 to 2015. Nellore (Bos indicus) growing bulls (Exps. 1, 2, and 3) and heifers (Exp. 4) were used in the feedlot trials. In all experiments, animals were fed for ad libitum DMI. The WI, animal performance, diet composition, and environmental data were collected. The prediction of WI using the current published WI equations was evaluated by regressing predicted and measured WI values. The regression was evaluated using the two-hypothesis test: H0: β0 = 0 and H0: β1 = 1 and Ha: not H0. If both null hypotheses were not rejected, it was concluded that the tested equation accurately estimated WI. To develop a WI prediction equation based on the input variables, a leave-oneout cross-validation method was proposed. The proposed equation was evaluated using similar methodology described above. All previously published eight equations overestimated WI of cattle used in the four experiments conducted in southeast Brazil. A possible explanation for the overestimate of WI is that previously published WI equations were generated from data collected from predominantly Bos taurus cattle raised under temperate climates. From the data collected from experiments conducted with Nellore cattle in southeast Brazil, the proposed equation (WI = 9.449 + 0.190 × MBW + 0.271 × T MAX −0.259 × HU + 0.489 × DMI, where the MBW is the metabolic BW (kg 0.75 ), T MAX is the maximum temperature (°C), HU is the humidity (%) and DMI in kg/d), more accurately to predicts WI of cattle raised under tropical conditions.
Effects of dietary crude protein (CP) supply on intake, digestibility, performance, and N balance were evaluated in young Nellore bulls consuming static or oscillating CP concentrations. Forty-two young bulls (initial BW of 260 ± 8.1 kg; age of 7 ± 1.0 mo) were fed ad libitum and were randomly assigned to receive one of six diets with different CP concentrations for 140 d: 105 (LO), 125 (MD), or 145 g CP /kg DM (HI), and LO to HI (LH), LO to MD (LM), or MD to HI (MH) oscillating CP at a 48-h interval for each feed. At the end of the experiment, bulls were slaughtered to evaluate carcass characteristics. Linear and quadratic effects were used to compare LO, MD, and HI, and specific contrasts were applied to compare oscillating dietary CP treatments vs. MD (125 g CP/kg DM) static treatment. Dry matter intake (DMI) was not affected (P > 0.26) by increasing or oscillating dietary CP. As dietary N concentration increased, there was a subsequent increase in apparent N compounds digestibility (P = 0.02), and no significant difference (P = 0.38) was observed between oscillating LH and MD. Daily total urinary and fecal N increased (P < 0.01) in response to increasing dietary CP. Significant differences were observed between oscillating LM and MH vs. MD, where bulls receiving the LM diet excreted less (P < 0.01; 71.21 g/d) and bulls fed MH excreted more (P < 0.01) urinary N (90.70 g/d) than those fed MD (85.52 g/d). A quadratic effect was observed (P < 0.01) for retained N as a percentage of N intake, where the bulls fed LO had greater N retention than those fed HI, 16.20% and 13.78%, respectively. Both LH and LM had greater (P < 0.01) daily retained N when compared with MD. Performance and carcass characteristics were not affected (P > 0.05) by increasing or oscillating dietary CP. Therefore, these data indicate that although there is no alteration in the performance of growing Nellore bulls fed with oscillating CP diets vs. a static level of 125 g CP/kg DM, nor static low (105 g CP/kg DM) and high (145 g CP/kg DM) levels; there may be undesirable increases in environmental N excretion when the average dietary CP content is increased. The results suggest that dietary CP concentrations of 105, 125 g/kg DM, or within this range can be indicated for finishing young Nellore bulls, since it reaches the requirements, reduces the environmental footprint related to N excretion, and may save on costs of high-priced protein feeds.
One hundred and forty-four Angus x Simmental steers were allotted by body weight (BW; 363 kg), breed composition, and farm origin to a 3 x 2 factorial arrangement of 6 treatments (4 pens per treatment) to determine the effect of Mootral (garlic + citrus extract; 0.25% of the diet DM vs. 0.0%) on methane emissions, growth and carcass characteristics of feedlot cattle. During the first 84 days, cattle were fed three different forage concentrations in the diet (15, 41.5, or 68% corn silage) with or without Mootral. From day 85 to slaughter, corn silage was included at 15% of the diet DM with or without Mootral. Methane emissions were measured on day 42 to 46 and day 203 to 207. Data were analyzed using the GLIMMIX procedure of SAS. Mootral did not affect methane emissions on days 42 to 46 (P ≥ 0.47), but there was a forage effect, where steers fed the 68% corn silage emitted more methane on a g/d (P = 0.05) and a g/kg of DMI (P = 0.007) basis and tended (P = 0.07) to produce more methane on g/kg BW basis compared to steers fed the 15% corn silage diet. On d 203 to 207, steers fed Mootral emitted less (P ≤ 0.03) methane on a g/d, g/kg DMI, and g/kg BW basis compared to steers not fed Mootral. There was an interaction (P = 0.03) between forage concentration and Mootral for DMI from d 0 to 84, where Mootral decreased DMI of steers fed 15% corn silage but did not affect DMI of steers fed 41.5 or 68% corn silage. There were no effects (P ≥ 0.22) of forage concentration or Mootral on BW or average daily gain at any time, or on DMI from d 84 to slaughter and overall. However, overall calculated NEm and NEg tended to be greater for steers fed Mootral (P ≤ 0.10). Intake from d 0-84 was lower and gain:feed from d 0-84 and overall was greater (P = 0.04) for steers fed 68% compared to steers fed 41.5% corn silage. Calculated NEm and NEg from day 0 to 84 and overall were greater for steers fed 68% corn silage compared to steers fed 41.5% corn silage (P ≤ 0.03). Mootral tended to decrease (P ≤ 0.09) fat thickness and yield grade. In conclusion, increasing forage concentration increased methane emissions and Mootral decreased methane production in 15% corn silage diets and tended to improve carcass leanness.
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