BackgroundBiodiversity surveys have long depended on traditional methods of taxonomy to inform sampling protocols and to determine when a representative sample of a given species pool of interest has been obtained. Questions remain as to how to design appropriate sampling efforts to accurately estimate total biodiversity. Here we consider the biodiversity of freshwater ostracods (crustacean class Ostracoda) from the region of Churchill, Manitoba, Canada. Through an analysis of observed species richness and complementarity, accumulation curves, and richness estimators, we conduct an a posteriori analysis of five bioblitz-style collection strategies that differed in terms of total duration, number of sites, protocol flexibility to heterogeneous habitats, sorting of specimens for analysis, and primary purpose of collection. We used DNA barcoding to group specimens into molecular operational taxonomic units for comparison.ResultsForty-eight provisional species were identified through genetic divergences, up from the 30 species previously known and documented in literature from the Churchill region. We found differential sampling efficiency among the five strategies, with liberal sorting of specimens for molecular analysis, protocol flexibility (and particularly a focus on covering diverse microhabitats), and a taxon-specific focus to collection having strong influences on garnering more accurate species richness estimates.ConclusionsOur findings have implications for the successful design of future biodiversity surveys and citizen-science collection projects, which are becoming increasingly popular and have been shown to produce reliable results for a variety of taxa despite relying on largely untrained collectors. We propose that efficiency of biodiversity surveys can be increased by non-experts deliberately selecting diverse microhabitats; by conducting two rounds of molecular analysis, with the numbers of samples processed during round two informed by the singleton prevalence during round one; and by having sub-teams (even if all non-experts) focus on select taxa. Our study also provides new insights into subarctic diversity of freshwater Ostracoda and contributes to the broader “Barcoding Biotas” campaign at Churchill. Finally, we comment on the associated implications and future research directions for community ecology analyses and biodiversity surveys through DNA barcoding, which we show here to be an efficient technique enabling rapid biodiversity quantification in understudied taxa.

Polar bears are important socio-cultural symbols in the communities of the Eeyou Marine Region (EMR) in northwestern Québec, Canada. Members of the Cree communities in this region are generally not active polar bear hunters, but they encounter polar bears when fishing, trapping, or hunting during the ice-free season. A growing body of scientific evidence suggests that reduced annual sea ice cover in Hudson Bay has led to declines in body condition of polar bears in the local Southern Hudson Bay subpopulation and to a population decline in the neighboring Western Hudson Bay subpopulation. In June 2012, we conducted 15 semi-directed interviews on the subject of polar bear biology and climate change with local elders and hunters in three communities in the northern EMR: Wemindji, Chisasibi, and Whapmagoostui. The interviews held in Whapmagoostui included informants from Kuujjuarapik, the adjacent Inuit community. The interviews addressed knowledge gaps in the Recovery Strategy for Polar Bear in Ontario. Transcripts of the interviews were coded thematically and analyzed using both qualitative and quantitative methods. The interviews revealed important insights into polar bear distribution, terrestrial habitat use, denning, and foraging patterns. Participants were unanimous in their recognition of a warming climate and prolonged ice-free season in the area in recent years. However, communities differed in their observations on other issues, with latitudinal trends evident in observations of polar bear distribution, denning activity, and foraging habits. Communities also differed in their perception of the prevalence of problem polar bears and the conservation status of the species, with one-third of participants reporting that polar bears will be unaffected by, or even benefit from, longer ice-free periods. A majority of participants indicated that the local polar bear population was stable or increasing. Interviewees also identified future research priorities pertinent to the communities, and provided comments on the methods employed by polar bear biologists. Our results demonstrate that communities in the EMR have important knowledge about polar bear ecology and illustrate the unique opportunities and challenges of combining traditional ecological knowledge with wildlife science in the context of a rapidly changing subarctic environment.