Objectives Lockdown measures have been adopted in many countries around the world to control the spread of COVID-19. These measures induced long confinement period that may have had an unintended negative impact on children's life behaviors and health. This study aimed to investigate the impact of behaviors related to COVID-19 confinement on body weight/BMI changes in children from Constantine, Algeria. Methods This was a cross-sectional survey based on children aged 5 to 12 years dwelling in any province of Constantine. Parents of children completed an online-distributed questionnaire at two time points (between April and May and anther time between 11th July and 10th august 2020). The questionnaire assessed sociodemographic information, anthropometric data, physical activity, dietary, eating habits, and other factors related to children’s lifestyle before and during lockdown. Paired before and during lockdown comparison for each lifestyle multiple statistic tests were performed to assess associations among before and during lockdown data. Results 275 questionnaires were completed (59.7%). Mean self-reported weight and BMI significantly increased by 1.43 kg and 0.84 kg/meter 2, respectively. Among the examined variables, increased unhealthy food consumption, snacks, number of daily meals taken, low physical activity and increased sedentary behaviors were significantly correlated with higher body weight gain (P < 0.005). These behaviors may not only have a significant impact on the development of childhood obesity but also on the deterioration of the mental state of the children surveyed. Conclusion Restrictive measures during the COVID-19 lockdown, and home confinement, school closure makes children more vulnerable to environmental risks. Results from this study highlight the risk associated with a shift in eating habits, increased dietary intake, decreased physical activity, increased sedentary behaviors, and their impact in exacerbating the gain in body weight and BMI. Supplementary Information The online version contains supplementary material available at 10.1007/s10995-022-03441-2.

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Enzymes from halophilic fungi offer interesting biotechnological applications, which lead us to explore novel producing strains. 23 fungi were isolated from Algerian saline soil. Among the three strains presenting laccase activities, one exhibited the high decolourising capacity of olive mill wastewaters. Identification showed that the efficient isolate GS15 belongs to Penicillium chrysogenum. This strain achieves optimal growth at 15% NaCl, 25 °C, pH 5, dark, aerobic and static conditions. The selected fungus is capable of producing extracellular enzymes as follows: caseinase, tannase, esterase and lipase. The laccase activities produced by P. chrysogenum on raw olive wastes are being reported here for the first time. GS15 produced 183.0 and 203.0 U/L of laccase activities in 10% and 20% unsupplemented olive mill wastewaters, respectively. The significant enzymatic activities can be correlated to the high ability of GS15 to decolourise industrial wastewater from the olive oil extraction. In these conditions no pre-treatment of olive wastewaters was needed. On the untreated grinded and non-grinded olive pomace, the laccase activity was 5.78 U/g and 5.36 U/g, respectively. Because the halophilic fungus has basic requirement for growth, this fungal strain is promising for saline biotechnological applications.

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