Summary. Forty-six, mature, New Zealand white does were used for collection of fluid from the Fallopian tubes. Ligation of the tubes into four isolated segments was carried out with minimal disturbance to the blood supply, using an operating microscope. The volume of fluid collected decreased from the fimbriated end to the utero-tubal junction.Analysis of the chemical constituents of the fluid from the first three segments showed that the concentration of sodium, bicarbonate, inorganic phosphate, proteins and lactic acid increased significantly from the fimbriated end to the utero-tubal junction, while chloride concentration decreased significantly. These results support the possibility that the different segments of the oviduct may play specific roles in early events of reproduction.