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Microalgae perform oxygenic photosynthesis and are capable of taking up a large amount of CO 2 , using an inducible CO 2 concentrating mechanism (CCM), and fixing CO 2 into higher compounds. These characteristics make the microalgae potentially useful for removal and utilization of CO 2 emitted from industrial plants and, generally, the usage of photosynthetic microorganisms has increased and significantly improved as a solution for CO 2 emissions. In this light and based on previous research using ^å~Ä~Éå~=ÅóäáåÇêáÅ~ IAM M1 and péáêìäáå~=éä~íÉåëáë IAM M 135, enhancement was sought for CO 2 fixation and biomass production by `ÜäçêÉää~=îìäÖ~êáë=Buitenzorg by increasing the photon flux density concurrent with increases in culture biomass during the cellular growth phase and was compared to cultures of `ÜäçêÉää~ grown at optimal constant illumination, with all cultures grown using Bennick basal medium, 29°C, and a flow of 1.0 atm. 10% CO 2 enriched air delivered to three in serial photobioreactors of 0.200 dm 3 capacity each. The results showed that increasing illumination during culture increased biomass production of `ÜäçêÉää~ by ~60% as well as increased CO 2 fixation ability by ~7.0%. It was also demonstrated that the non-competitive inhibition of [HCO 3 − ] as a carbon source significantly affected the cultivation in both the increasing and constant photon flux density regimes. © KSBB hÉóïçêÇëW=Äáçã~ëëI=Chlorella vulgaris=_ìáíÉåòçêÖI=~äíÉê~íáçåI=éÜçíçÄáçêÉ~Åíçê= = = = =