The process hygiene criterion is a microbiological criterion that applies to the process offood production and processing and indicates the proper functioning of the production process byrepresenting the value of the contamination above which corrective measures are taken to maintainthe process hygiene. Pasteurized milk is a product obtained by heat treatment of raw milk at atemperature of 63° C for 30 minutes or 72° C for 15 seconds. Pasteurization has two purposes, thefirst one is to elimination of all pathogenic microorganisms, and the second is to reduce the numberof saprophytic microorganisms, which prolongs the shelf life without changing the nutritional andbiological value of the milk.The aim of the study is to examine the safety of pasteurized milk and the conditions of hygiene inthe production process based on the results of the examination of pasteurized milk on the criteria ofhygiene in the production process, as well as to develop a proposal for the recommendedmicroorganisms to be tested in the process of the production of pasteurized milk.Samples of pasteurized milk come from a pasteurizing plant that purchases milk from the territoryof Republika Srpska, and were sampled in the six-month period (January-June), within self-controland official control. For microbiological testing of raw milk, standard BAS ISO methods were used.The results of pasteurized milk testing in relation to the hygiene criteria in the production processare satisfactory in relation to the Rulebook on Microbiological Criteria for Food. The results of selfcontrolon the recommended microbiological criteria in the process of obtaining pasteurized milk,which are given in the Guideline on the Application of Microbiological Criteria for Food, aresatisfactory in relation to the finding of Listeria monocytogenes, Salmonella spp. and coagulasepositive staphylococci, and unsatisfactory for a number of microorganisms.The results of official controls in the production process of pasteurized milk are unsatisfactory dueto the increased number of microorganisms in 12.50% of the samples. 22.20% of pasteurized milksamples had the number of microorganisms larger than 105CFU/ml within self-control and officialcontrols. Examination of pasteurized milk in the self-control of the production process should beperformed on enterobacteria and the total count of microorganisms, and in the interpretation of theresults the recommended limit values from the Guideline on the application of microbiologicalcriteria for food should be used.
Uncontrolled use of antimicrobials in the prophylaxis and treatment of human and animal diseases leads to the appearance of residues in the environment and food. The use of antimicrobials as growth promoters in breeding affirms the importance of their residual finding in foods of animal origin. Bacteria of the genus Salmonella are one of the most common causes of food poisoning. All species of this genus are pathogenic to humans and cause various diseases known as salmonellosis. Humans can become infected through the faecal-oral route by consuming contaminated food and water or by direct contact with an animal. Salmonella that are resistant to antimicrobial drugs can transfer resistance genes to other microorganisms. In this work, the resistance of 10 Salmonella isolates from poultry meat to 8 different antimicrobial substances was examined by the disk diffusion method. All Salmonella isolates were sensitive to trimethoprim-sulfamethoxazole and chloramphenicol. All isolates were resistant to amoxicillin and significant percentages were resistant to other antimicrobial drugs. Also, multi-drug resistance of Salmonella isolates was found. The best prevention of salmonellosis in humans is constant and comprehensive control of this hazard in food products during production, processing, storage, and sale.
Sjenica cheese is an artisanal cheese stored in brine, traditionally produced from raw sheep's milk in the southern part of Serbia -Sjenica Pester plateau.The aim of this study was to perform the safety evaluation of Sjenica cheese. As one of the safety criteria we considered the number of coagulase positive staphylococci and their enterotoxigenic potential. Antibiotic susceptibility/resistance patterns of autochthonous lactic acid bacteria and coagulase-positive staphylococci isolated from Sjenica cheese was also investigated.During the monitoring period of the cheese-making process, coagulase positive staphylococci did not reach the value of 10 5 cfu/g. Among coagulase positive staphylococci, 12 (46,15%) isolates showed enterotoxigenic potential and were identifi ed as Staphylococcus intermedius (11 isolates) and Staphylococcus aureus (1 isolate). Vancomycin resistance was the most prevalent phenotypic resistance profi le in coagulase positive staphylococci.Lactococci present the most dominant population among lactic acid bacteria. The most prevalent resistance phenotype in lactococci was resistance to streptomycin (83.33%), ampicillin and penicillin (70.83%); lactobacilli were characterized by resistance to vancomycin (62.5%) and tetracycline (54.17%), while resistance to streptomycin (82.46%) was the most prevalent phenotypic profi le in enterococci.All coagulase positive staphylococci and lactic acid bacteria isolates that showed resistance to tetracycline on disc diffusion and E-test, were tested for the presence of ribosomal protection proteins, tet(M) and tet(K) genes. All isolates were positive for ribosomal protection proteins genes; 14 (60.87%) isolates showed tet(M) gene presence, while 2 lactobacilli isolates revealed the presence of tet(K) gene.
Health-safe water is the basis of a healthy life and is one of the priorities in primary health care. Water safety involves microbiological, physicochemical and radiologically clean water, a sufficient amount of water and continuous delivery. Due to the great epidemiological importance of water, whose impact is immediate and through which various infectious diseases can be transmitted harmful and dangerous chemicals can be introduced, it is necessary, in order to protect human health, to control the safety of drinking water. The aim of this study was to determine the microbiological safety of water used for watering animals, as well as in the food industry. Based on data collected from year 2015 to 2017, 26.20% water samples did not meet requirements defined by Regulation on hygienic quality of drinking water. The greatest number of samples was unsatisfactory due to an increased number of microorganisms at the temperatures of 22°C (63.40%) and 37°C (54.90%), and when it comes to pathogenic microorganisms due to the presence of intestinal enterococci (58.80%). The lowest risk of the presence of coliforms and E. coli is in the water from the water supplying network, while it's much higher presence is in wells and in the water from the wellspring. The presence of intestinal enterococci is significant in all three types of drinking water, while the presence of sulphite-reducing clostridia was observed only in the wellspring water.
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