Determination of influence of lupin natural phenolic compounds on antibacterial properties of its seeds was carried out. Raw material were seeds of Lupinus albus, L. luteus, and L. angustifolius. The methods included the determination of the content of proteins, total phenolic compounds, free phenolic acids, and tannins as well as antibacterial properties with ethanol extracts. The content of total phenolic compounds was smaller in testas than in cotyledons and the highest levels are observed in bitter cultivars of Lupinus albus cv. Bac and L. angustifolius cv. Mirela. Lupin tannins mainly occurred in cotyledons of the white lupin, predominantly in the bitter cultivar Bac. Free phenolic acids were mainly found in testas. Only extracts from the testas displayed antibacterial properties, which excludes the possibility of alkaloid influence on the results. The results suggest that inhibition of test bacteria growth depended mainly upon the content of the total phenolic compounds.
Antioxidant activity of ethanolic extracts obtained from two amaranth species was evaluated in a beta-carotene-linoleic acid model system. The addition of amaranth extracts in the range of 0.01-0.1% inhibited degradation of a beta-carotene in a model emulsion during incubation at 60 degrees C; 0.05% addition of amaranth seeds extract was proposed as practically applicable. The total content of phenolic compounds was estimated by the Folin-Ciocalteu method and ranged from 39.17 mg/100 g of Amaranthus caudatus to 56.22 mg/100 g of A. paniculatus seeds. Free phenolic acids contained in ethanolic extracts of amaranth seeds were purified and isolated by solid-phase extraction (SPE) and identified by reversed-phase high-performance liquid chromatography (RP-HPLC). The technique involved gave a good separation of the free phenolic acids in the amaranth seeds. Significant differences in phenolic acids profiles of both amaranth species were observed.
Ensuring food safety for people suffering fromvarious intolerances faces serious threats posed by allergenswhich have become a challenge for food manufacturers andmass caterers. These businesses are required to developand implement appropriate programs to manage allergenspresent in their sites. The purpose of this paper was to analyzethe actions taken by food manufacturers with respect tosubstances and products causing allergies or food intolerancesin the context of providing consumers with a safe, properlylabeled product. To achieve the objective of this study, a dataanalysis and synthesis method was employed based on requirementsdefined in obligatory and non-obligatory standardsfor food safety assurance and management in the foodproduction sector. In summary, as regards allergen management,the companies must create appropriate conditions andtake adequate measures (including the analysis of risks due tointentional and adventitious presence of allergens in food, andthe implementation of appropriate preventive actions). Solutionsprovided in the mandatory standards, mainly GHP andHACCP, as well as in non-mandatory standards, such as ISO22000, BRC, IFS and SQF, may be helpful in the managementof substances causing allergies or intolerances.
Celem pracy była ocena właściwości przeciwutleniających oraz zawartości związków fenolowych naparów herbatek będących suplementami diety. Analizie poddano 15 herbatek ziołowych oraz ziołowoowocowych, które podzielono na 5 grup, przyjmując za kryterium deklarację wpływu odżywczego i fizjologicznego określoną przez producenta -wpływ na włosy i skórę, figurę, samopoczucie, odporność oraz energię ciała. Zakres badań obejmował: oznaczenie właściwości przeciwrodnikowych (test z rodnikiem DPPH), oznaczenie zawartości związków fenolowych (metoda Folina-Ciocalteu'a) oraz oznaczenie siły redukującej (metoda FRAP).Na podstawie przeprowadzonych badań stwierdzono, że napary herbatek wykazały zróżnicowane właściwości przeciwutleniające oraz zawartości związków fenolowych. Grupa herbat wpływających na odporność charakteryzowała się najwyższą aktywnością przeciwrodnikową, najwyższą siłą redukującą oraz najwyższą zawartością polifenoli ogółem. Wyniki badań ukazują dodatnią korelację między aktywnością przeciwutleniającą wyrażoną odsetkiem wygaszonego rodnika DPPH, siłą redukującą FRAP oraz zawartością związków fenolowych ogółem.
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