Norway spruce Picea abies is one of Europe’s most economically important tree species. However, it is highly susceptible to the root rot fungus Heterobasidion parviporum. Climate change will benefit the pathogen as the tree host is weakened by, e.g., extended drought. Breeding can improve forest health, and several root rot genetic markers are suggested to improve the resistance of Norway spruce. This study aimed to compare the resistance (here defined as necrosis length) of Norway spruce families and genotypes against two strains of H. parviporum under different water availabilities. Our results show that the family and the genotype within the family have an impact on the necrosis length that is related to the aggressiveness of the fungal strains. Under low water conditions, the necrosis increased only in horizontal directions in phloem and sapwood. Similarly, the growth (seedling height) was not disturbed by abiotic stress (less water), indicating that the stress level (drought) was too low in this setting. The knowledge gained in this study could improve forest health in the changing climate by understanding the response of Norway spruce to pathogenic attacks under additional stress at the family level. This knowledge could be strategically used in forest breeding to improve the resistance of Norway spruce trees against root rot.
The major threats to the sustainable supply of forest tree products are adverse climate, pests and diseases. Climate change, exemplified by increased drought, poses a unique threat to global forest health. This is attributed to the unpredictable behavior of forest pathosystems, which can favor fungal pathogens over the host under persistent drought stress conditions in the future. Currently, the effects of drought on tree resistance against pathogens are hypothetical, thus research is needed to identify these correlations. Norway spruce (Picea abies (L.) H. Karst.) is one of the most economically important tree species in Europe and is considered highly vulnerable to changes in climate. Dedicated experiments to investigate how disturbances will affect the Norway spruce—Heterobasidion sp. pathosystem are important, in order to develop different strategies to limit the spread of H. annosum s.l. under the predicted climate change. Here, we report a transcriptional study to compare Norway spruce gene expressions to evaluate the effects of water availability and the infection of Heterobasidion parviporum. We performed inoculation studies of three-year-old saplings in a greenhouse (purchased from a nursery). Norway spruce saplings were treated in either high (+) or low (−) water groups: high water group received double the water amount than the low water group. RNA was extracted and sequenced. Similarly, we quantified gene expression levels of candidate genes in biotic stress and jasmonic acid (JA) signaling pathways using qRT-PCR, through which we discovered a unique preferential defense response of H. parviporum-infected Norway spruce under drought stress at the molecular level. Disturbances related to water availability, especially low water conditions can have negative effects on the tree host and benefit the infection ability of the pathogens in the host. From our RNA-seq analysis, 114 differentially expressed gene regions were identified between high (+) and low (−) water groups under pathogen attack. None of these gene pathways were identified to be differentially expressed from both non-treated and mock-control treatments between high (+) and low (−) water groups. Finally, only four genes were found to be associated with drought in all treatments.
Norway spruce Picea abies is one of Europe's most economically important tree species. However, it is highly susceptible to the root rot fungus Heterobasidion parviporum. Climate change will benefit the pathogen as the tree host is weakened by, e.g., extended drought. Breeding can improve forest health, and several root rot genetic markers are suggested to improve the resistance of Norway spruce. This study aimed to compare the resistance (here defined as necrosis length) of Norway spruce families and genotypes against two strains of H. parviporum under different water availabilities. Our results show that the family and the genotype within have an impact on the necrosis length that is related to the aggressiveness of the fungal strains. Under low water conditions, the necrosis increased only in horizontal directions in phloem and sapwood. Similarly, the growth was not disturbed by abiotic stress (less water), indicating that the stress level (drought) was too low in this setting. The knowledge gained in this study could improve forest health in the changing climate by understanding the response of Norway spruce to pathogenic attacks under additional stress at the family level. This knowledge could be strategically used in forest breeding to improve the resistance of Norway spruce trees against root rot.
The major threats to the sustainable supply of forest tree products are adverse climate, pests and diseases. Climate change, exemplified by increased drought, poses a unique threat to global forest health. This is attributed to the unpredictable behavior of forest pathosystems, which can favor fungal pathogens over the host under persistent drought stress conditions in the future. Currently, the effects of drought on tree resistance against pathogens are hypothetical, thus research is needed to identify these correlations. Norway spruce (Picea abies) is one of the most economically important tree species in Europe, and is considered highly vulnerable to changes in climate. Dedicated experiments to investigate how disturbances will affect the Norway spruce - Heterobasidion sp. pathosystem are important, in order to develop different strategies to limit the spread of H. annosum s.l. under the predicted climate change. Here, we report a transcriptional study to compare Norway spruce gene expressions to evaluate the effects of water availability and the infection of Heterobasidion parviporum. We performed inoculation studies of three-year-old saplings in a greenhouse (purchased from a nursery). Norway spruce saplings were treated in either high (+) or low (-) water groups: high water group received double the water amount than the low water group. RNA was extracted and sequenced. Similarly, we quantified gene expression levels of candidate genes in biotic stress and jasmonic acid (JA) signaling pathways using qRT-PCR, through which we discovered a unique preferential defense response of H. parviporum-infected Norway spruce under drought stress at the molecular level. Disturbances related to water availability, especially low water conditions can have negative effects on the tree host and benefit the infection ability of the pathogens in the host. From our RNA-seq analysis, 114 differentially expressed gene regions were identified between high (+) and low (-) water groups under pathogen attack. None of these gene pathways were identified to be differentially expressed from both non-treated and mock-control treatments between high (+) and low (-) water groups. Finally, only four genes were found to be associated with drought in all treatments.
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