KB cells express a folate-binding protein that is anchored to the plasma membrane by a glycosylated phosphatidylinositol (GPI) tail and these cells can grow in medium containing a very low folate concentration (1 nM). In contrast, mouse 3T3 cells do not express a membrane-associated folate-binding protein and cannot grow under similar low folate conditions. In these studies, 3T3 cells were transfected with a vector containing the cDNA that codes for the KB cell folate-binding protein. In contrast to the wild-type 3T3 cells, the transfected 3T3 cells express a level of folate-binding protein similar to KB cells, 1 and 1.4 ng/,ug protein, respectively. The capacity for binding 13HIfolate to the surface of transfected 3T3 cells cultured in folatedeficient medium is 7.7 pmol/ 106 cells, and this is -50% of the surface binding capacity of KB cells under similar culture conditions. Moreover, after treatment of the transfected 3T3 cells with phospholipase C specific for phosphatidylinositol, the binding of 13HI folate to the surface of these cells is reduced by 90%, indicating that, like the KB cells, the folate-binding protein is anchored to the plasma membrane by a GPI tail. Although the doubling time of wild-type 3T3 cells markedly increases after 13 d of culture in folate-deficient medium, the doubling time of both the transfected 3T3 cells and KB cells do not change. The results of these experiments indicate that the GPI-anchored folate-binding protein provides a mechanism to maintain a level of folate that permits the folate-dependent metabolic functions necessary for cell survival under low folate conditions. (J. Clin. Invest. 1992. 90:840-847.)
Online trolling is aggressive online behavior that has severe consequences for the mental health of internet users. Online trolling can be influenced by personal factors and psychological states. Based on the General Aggression Model, moral disengagement was examined as a moderator of the direct and indirect relations between mindfulness and online trolling via social media fatigue. A total of 1123 college students completed questionnaires regarding their experience with online trolling, mindfulness, social media fatigue, and moral disengagement. The results showed that individuals with high mindfulness were less likely to troll others online, which was mediated by their social media fatigue. Furthermore, moral disengagement moderated the indirect relationship between mindfulness and online trolling. Specifically, the relationship between mindfulness and social media fatigue became weaker for individuals with high moral disengagement. The relationship between social media fatigue and online trolling became strengthened for individuals with high moral disengagement. These findings elucidate the role of personal factors and the present internal state in online trolling and suggest that comprehensive intervention programs may be promising for reducing online trolling.
Purpose
With the rise of social media, an increasing number of young females have focused more attention on their body image, leading to social appearance anxiety. Thus, the current study aimed to investigate a moderated mediation model of social appearance anxiety and online impulse purchases of fashionable outfits through self-control moderated by subjective socioeconomic status during the pandemic.
Methods
A total of 1651 female college students (
Age
= 17–24,
M
age
= 19.30,
SD
= 1.14) with more than one month of closed-off management experience completed self-report questionnaires concerning social appearance anxiety, self-control, online impulse purchases of fashionable outfits, and subjective socioeconomic status.
Results
After controlling for the potential influence of coronavirus stress, the results indicated that social appearance anxiety was positively related to online impulse purchases of fashionable outfits among female college students during the pandemic, mediated by self-control. Furthermore, subjective socioeconomic status moderated the indirect link between social appearance anxiety and the online impulse purchase of fashionable outfits. Specifically, subjective socioeconomic status buffers the negative effect of social appearance anxiety on self-control and the risk effect of low self-control on the online impulse purchases of fashionable outfits.
Implications
The current study deepens the research on the relationship between social appearance anxiety and online impulse purchases during pandemic periods; meanwhile, it provides evidence for preventing excessive online impulse purchases of fashionable outfits among young females.
The mental health of vocational college students has been neglected during the COVID-19 pandemic. Prospective imagery may play a role in the relationships among stress, anxiety and depression. This study aimed to survey the mental health of Chinese vocational college students and explore the mediation effect of prospective imagery vividness and anxiety symptoms on the relationship between perceived stress and depressive symptoms. A total of 2, 381 vocational college students (
M
age
= 18.38 years, range: 16–21,
SD
= 0.92) provided self-report data on perceived stress, anxiety and depressive symptoms and prospective imagery vividness. Two potential serial mediation models were postulated regarding the roles of prospective imagery vividness and anxiety symptoms in the relationship between perceived stress and depressive symptoms. The prevalence rates of stress, anxiety and depressive symptoms were 55.7%, 33.2% and 53.5%, respectively, among vocational college students. Perceived stress was associated with decreased vividness of positive prospective imagery and increased vividness of negative prospective imagery and anxiety symptoms, leading to increased depressive symptoms. Additionally, prospective imagery vividness and anxiety symptoms had a serial mediation effect on the relationship between perceived stress and depressive symptoms. The results demonstrated that impoverished vividness of positive prospective imagery is not only a central feature of depression but also associated with anxiety. Interventions targeting prospective imagery vividness may alleviate anxiety and depressive symptoms among Chinese vocational college students and should be implemented as soon as possible during the COVID-19 pandemic.
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