The brown planthopper (BPH) Nilaparvata lugens is one of the most destructive insect pests in the rice fields of Asia. Like other hemipteran insects, BPH is not susceptible to Cry toxins of Bacillus thuringiensis (Bt) or transgenic rice carrying Bt cry genes. Lack of Cry receptors in the midgut is one of the main reasons that BPH is not susceptible to the Cry toxins. The main Cry-binding proteins (CBPs) of the susceptible insects are cadherin, aminopeptidase N (APN), and alkaline phosphatase (ALP). In this study, we analyzed and validated de novo assembled transcripts from transcriptome sequencing data of BPH to identify and characterize homologs of cadherin, APN, and ALP. We then compared the cadherin-, APN-, and ALP-like proteins of BPH to previously reported CBPs to identify their homologs in BPH. The sequence analysis revealed that at least one cadherin, one APN, and two ALPs of BPH contained homologous functional domains identified from the Cry-binding cadherin, APN, and ALP, respectively. Quantitative real-time polymerase chain reaction used to verify the expression level of each putative Cry receptor homolog in the BPH midgut indicated that the CBPs homologous APN and ALP were expressed at high or medium-high levels while the cadherin was expressed at a low level. These results suggest that homologs of CBPs exist in the midgut of BPH. However, differences in key motifs of CBPs, which are functional in interacting with Cry toxins, may be responsible for insusceptibility of BPH to Cry toxins.
Background: Short-segment internal fixation with intermediate straightforward monoaxial screws (SSIF-SFM) and long-segment internal fixation (LSIF) are the two major surgical options for thoracolumbar (TL) fracture. However, SSIF-SFM might not provide adequate support to the spine, and LSIF is unnecessarily extensive. SSIF with intermediate inclined-angle polyxial screw (SSIF-IAP) might offer an alternative solution for the treatment of TL fracture. Methods: A retrospective study was conducted. Sixty-nine patients (47 males and 22 females; average 34.5 years) with Denis type B TL fracture who met the criteria for inclusion were enrolled. Sagittal Cobb's angle (SCA), anterior vertebral body height (AVBH), vertebral body index (VBI), and spinal canal encroachment (SCE) were measured and assessed. Visual analogue scale (VAS) and Oswestry disability index (ODI) were also evaluated. Results: The average values of incision length, blood loss, duration of operation, and hospital stay in the SSIF-IAP group and SSIF-SFM group were significantly decreased compared with those in the LSIF group. The AVBH and VBI in the SSIF-IAP group and LSIF group were significantly improved than those in the SSIF-SFM group at 6-month and the latest follow-ups (P < 0.05). The correction losses of AVBH and VBI (calculated by the reduction of AVBH and VBI) in the SSIF-IAP group and LSIF group were also significantly decreased compared with those in the SSIF-SFM group at 6-month and the latest follow-ups (P < 0.05). There was no significant difference of SCE among the three groups postoperatively. The VAS and ODI in the SSIF-IAP group and SSIF-SFM group were significantly decreased compared with those in the LSIF group at 6-month and the latest follow-ups (P < 0.05).
The fabrication of MCL and the mechanism of MCL-mediated antitumor effects against melanoma.
Background: Short-segment internal fixation with intermediate straight-forward monoaxial screws (SSIF-SFM) and long-segment internal fixation (LSIF) are the tow major surgical options for thoracolumbar (TL) fracture. However, SS IF-SFM might not provide adequate support to the spine and LSIF is un necessarily extensive. SSIF with intermediate inclined-angle polyxial screw (SSIF-IAP) might offer an alternative solution for the treatment of TL fracture. Methods: A retrospective study was conducted. 69 patients (47 males and 22 females; average 34.5 years) with Denis type B TL fracture who met the criteria for inclusion were enrolled. Sagittal Cobb’s angle (SCA), anterior vertebral body height (AVBH), vertebral body index (VBI) and spinal canal encroachment (SCE) were measured and assessed. Visual analogue scale (VAS) and oswestry disability index (ODI) were also evaluated. Results: The average value of incision length, blood loss, duration of operation and hospital stay in the SSIF–IAP group and SSIF-SFM group were significantly decreased compared with those in the LSIF group. The AVBH and VBI in the SSIF-IAP group and LSIF group were significantly improved than those in the SSIF–SFM group at 6-month and the latest follow-ups (p < 0.05). The correction losses of AVBH and VBI (calculated by the reduction of AVBH and VBI) in the SSIF-IAP group and LSIF group were also significantly decreased compared with those in the SSIF–SFM group at 6-month and the latest follow-ups (P < 0.05). There was no significant difference of SCE among the three groups postoperatively. The VAS and ODI in the SSIF-IAP group and SSIF-SFM group were significantly decreased compared with those in the LSIF group at 6-month and the latest follow-ups (P < 0.05). Conclusion: Both SSIF- IAP and LSIF can improve the biomechanical stability as compared with SSIF -SFM . Moreover, SSIF-IAP was an effective and reliable operative technique for patients with Denis type B TL fracture.
Backgroud Short-segment internal fixation with straight-forward monoaxial screw (SSIF-SFM) and long-segment internal fixation (LSIF) are two major surgical options for thoracolumbar (TL) fracture, however, limitations of both surgical options l are obvious. SSIF with inclined-angle polyxial screw (SSIF-IAP) have been developed to take advantage of their benefits and minimize their adverse effects . Methods 69 consecutive patients (47 males and 22 females; average 34.5 years ) who met the criteria for inclusion were enrolled in this study . Sagittal Cobb’s angle (SCA), anterior vertebral body height (AVBH), vertebral body index (VBI) and spinal canal encroachment (SCE) were measured and assessed . Functional recovery Visual Analogue Scale (VAS) and Oswestry disability index (ODI) were also evaluated. Results The value of incision length, mean blood loss, duration of operation and hospital stay in the SSIF–IAP group and SSIF-SFM group were significantly lower than those in the LSIF group. The AVBH and VBI in the SSIF–IA P group and LSIF group were significantly more improved than those in the SSIF–SFM group at 6 months and the latest follow-ups (p < 0.05). The correction losses of AVBH and VBI ( calculated by the reduction of AVBH and VBI) in the SSIF-IAP group and LSIF group were also significantly lower than those in the SSIF–SFM group at 6 months and the latest follow-ups (P<0.05). There was no significant difference of SCE among three groups. The VAS and ODI in the SSIF–IAP group and SSIF-SFM group were significantly lower than those in the LSIF group at 6 months and the latest follow-ups (P<0.05). Conclusion SSIF-IAP can achieve comparable treatment outcomes compared with LSIF, but it was less invasive compared with LSIF . The SSIF–IA was an effective and reliable operative technique for patients with Denis type B TL fracture.
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