The most significant pulse crop in India is the mungbean [Vignar adiata (L.) Wilczek], which constitutes almost 16% of the national pulse area. For biotechnological interventions, it is prerequisite to develop a rapid and cost-effective method for genomic DNA isolation suitable for PCR. In present investigation,modified CTAB method was used to extract the genomic DNA from the leaf tissues of mungbesn. By crushing the leaves of several mungbean genotypes in a pre-heated CTAB extraction buffer, genomic DNA was extracted. Further, SSR markers were used to assess the efficiency of the extracted DNA.The SSR marker-based PCR amplification findings showed that the DNA extracted using this approach was of good quality and suitable for SSR analysis.
Mungbean (Vigna radiate L.) is one of the most important legume crops of Asiatic region. The average yield of mungbean is quite low due to its susceptibility against mungbean yellow mosaic virus (MYMV). Mungbean yellow mosaic virus disease (MYMD) is caused by MYMV, which is transmitted through whitefly (Bemisia tabaci). The controlling of this devastating disease is mainly depends upon spraying of insecticides, which cause serious ill effect on humans and soil health. Breeding for its resistance is one of the best strategies for developing MYMV resistant genotypes in mungbean. Several types of molecular markers have been used in marker assisted breeding (MAB) in mungbean. Among them SSR markers are widely used and a plethora of scientific advocate the use of the SSR marker in developing MYMV resistance in mungbean. Recent advancements in functional genomics and gene editing technologies can further enhance our understanding of the molecular mechanisms underlying resistance to MYMV and hence facilitate the development of MYMV resistant mungbean genotypes.
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