The Study evaluates effects of Seasonal Variation on Antioxidant Potential and Total Phenolic Content of Zanthoxylum armatum. DPPH and FRAP methods were used to evaluate effect of seasonal discrepancy on the antioxidant potential of plant Zanthoxylum armatum. The investigation observed that the antioxidant activity depends upon total phenolic and flavonoid contents which may affect by seasonal discrepancy. Study observed abundant of phenolic and flavonoid during summer season resulting potent antioxidant activities. The moderate antioxidant activity was observed in monsoon season. Study concluded that total phenolic content increased from rainy to summer season and thus antioxidant activities also vary with seasonal discrepancy.
Objective: The present investigation evaluated in vitro anti-inflammatory activity of Phyllanthus fraternus. Inhibition of Cyclooxygenase and 5-lipoxygenase was performed along with protein denaturation.Methods: Alcoholic extract of plant was subjected to in vitro anti-inflammatory activity and HPTLC analysis.Results: The results of anti-inflammatory activity showed significant inhibition in Cyclooxygenase and 5-lipoxygenase assay, extract also showed more than 70 % inhibition in protein denaturation method. HPTLC of plant materials was also performed; spots of alkaloids were recorded.Conclusion: Different alkaloids were spotted in chromatographic analysis and study suggested that anti-inflammatory activity of Phyllanthus fraternus may be due to the presence of alkaloids.Â
The study represents phytochemical standardization and antioxidant potential of ayurveda formulation Darvyadi Raskriya. The study involves development of high-performance thin-layer chromatographic (HPTLC) method for analysis of formulation. The study utilizes analysis of glycyrrhizin in formulation which is the phytoconstituent of Glycyrrhiza glabra one of the component of formulation. The sample in ethanol was applied on aluminium TLC plates using Linomat 5 spray (CAMAG). Linear ascending development was performed in twin trough glass chamber saturated with mobile phase. The mobile phase consisted of ethyl acetatemethanol-formic acid (10:5:1 v/v/v). The spectrodensitometric detection was performed at the wavelength of 254 nm. The regression analysis was found to be linear with r 2 =0.997 in the concentration range 5-25 ppm. The antioxidant activity of formulation was also found to be significant as compared to control.
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